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This work reports the development of electrospun poly(lactic acid) (PLLA) nanofiber membranes loaded with bovine lactoferrin (bLF) up to 20 wt%. The membranes present smooth and nondefective fibers with mean diameters between 717 \u00b1 197 and 495 \u00b1 127 nm, and an overall porosity of \u224880%. The hydrophobicity of the PLLA membranes is reduced by the presence of bLF. The release profile of bLF correlates with an anomalous transport model, with 17.7 \u00b1 3.6% being released over 7 weeks. The nanofiber mats show no cytotoxicity on human skin fibroblasts and even promote cell proliferation after short exposure periods. Furthermore, the developed membranes display antifungal activity against <jats:italic>Aspergillus nidulans<\/jats:italic> by inhibiting spore germination and mycelial growth. 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