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The aim of this study is to prepare and investigate the potential of swellable cross\u2010linked gelatin methacryloyl (c\u2010GelMA) microneedles (MNs) as a platform for minimally invasive extraction of interstitial skin fluid (ISF) toward straightforward point\u2010of\u2010care healthcare monitoring of renal complaints, by quantification of urea. c\u2010GelMA MNs are successfully prepared by photo\u2010cross\u2010linking and micromolding, faithfully replicating the master molds (387\u00a0\u00b1 16 \u00b5m height, 200 \u00b5m base and 500 \u00b5m tip\u2010to\u2010tip distance). These MN patches display good mechanical properties, withstanding more than 0.15 N per needle without breaking. Ex vivo skin insertion assays reveal that the MNs penetrate up to 237 \u00b5m depth, reaching the dermis, where they should extract ISF considering a real application. In an in vitro application using an agarose skin model system, the c\u2010GelMA MNs are able to efficiently recover urea (&gt;98%). Additionally, these MNs exhibit noncytotoxic effects toward human keratinocytes. These findings suggest that c\u2010GelMA MNs are promising devices for sampling ISF and offline analysis of urea, opening new avenues for simple point\u2010of\u2010care healthcare monitoring.<\/jats:p>","DOI":"10.1002\/mabi.202000195","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T03:33:34Z","timestamp":1598585614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":92,"title":["Swellable Gelatin Methacryloyl Microneedles for Extraction of Interstitial Skin Fluid toward Minimally Invasive Monitoring of Urea"],"prefix":"10.1002","volume":"20","author":[{"given":"Daniela F. 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