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First, functionalized few\u2010layer graphene aqueous suspensions are prepared from graphite by a stabilizer\u2010assisted liquid phase exfoliation process, using a pyrene derivative as stabilizer. Afterward, the films are produced and their physical, morphological, thermal, and mechanical properties are evaluated. Furthermore, their degradation and swelling profiles, as well as their biological behavior, are assessed. The incorporation of functionalized few\u2010layer graphene results in films with a nanolayered structure, lower roughness than the control CHI\/ALG films, and hydrophilic behavior. The mechanical characterization reveals an increase of the Young's modulus, ultimate tensile strength, and elongation at break due to the incorporation of the graphene derivative. A decrease in the electrical resistivity of the multilayered films is also observed. The biological assays reveal improved cytocompatibility toward L929 cells when functionalized few\u2010layer graphene is incorporated in the CHI\/ALG matrix. Therefore, these new graphene\u2010reinforced multilayered films exhibit interesting properties and great potential for biomedical applications, particularly in wound healing and cardiac and bone tissue engineering.<\/jats:p>","DOI":"10.1002\/mame.201700316","type":"journal-article","created":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T10:22:34Z","timestamp":1516184554000},"source":"Crossref","is-referenced-by-count":6,"title":["Nanostructured Biopolymer\/Few\u2010Layer Graphene Freestanding Films with Enhanced Mechanical and Electrical Properties"],"prefix":"10.1002","volume":"303","author":[{"given":"Cl\u00e1udia","family":"Silva","sequence":"first","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"},{"name":"Institute for Polymers and Composites\/i3N Department of Polymer Engineering University of Minho  4800\u2010058 Guimar\u00e3es Portugal"}]},{"given":"Sofia G.","family":"Caridade","sequence":"additional","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"}]},{"given":"Eunice","family":"Cunha","sequence":"additional","affiliation":[{"name":"Institute for Polymers and Composites\/i3N Department of Polymer Engineering University of Minho  4800\u2010058 Guimar\u00e3es Portugal"}]},{"given":"Maria P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"}]},{"given":"Helena","family":"Rocha","sequence":"additional","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"},{"name":"Institute for Polymers and Composites\/i3N Department of Polymer Engineering University of Minho  4800\u2010058 Guimar\u00e3es Portugal"}]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"}]},{"given":"Maria C.","family":"Paiva","sequence":"additional","affiliation":[{"name":"Institute for Polymers and Composites\/i3N Department of Polymer Engineering University of Minho  4800\u2010058 Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-4091","authenticated-orcid":false,"given":"Nat\u00e1lia M.","family":"Alves","sequence":"additional","affiliation":[{"name":"3B's Research Group Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark\u2010Parque de Ci\u00eancia e Tecnologia  4805\u2010017 Barco Taipas Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's Associate PT Government Laboratory  4710\u2010057 Braga\/Guimar\u00e3es Portugal"}]}],"member":"311","published-online":{"date-parts":[[2018,1,17]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2010.06.010"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1103\/RevModPhys.81.109"},{"volume-title":"Handbook of Carbon, Graphite, Diamond and Fullerenes","year":"1994","author":"Pierson H. 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