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This work aimed to evaluate the absorption of anthocyanins at the intestine using Caco\u20102 cells. Anthocyanin extract, rich in malvidin\u20103\u2010glucoside, was obtained from red grape skins and tested on Caco\u20102 cells. The absorption of anthocyanins, in absence or presence of 1% ethanol, was detected by HPLC\/DAD\/LC\u2010MS. Our results showed that this transport was significantly increased in the presence of ethanol especially after 60\u2009min of incubation. In addition, cells that were pretreated for 96\u2009h with anthocyanins (200\u2009\u03bcg\/mL) showed an increase of their own transport (about 50% increase). Expression of glucose transporters sodium\u2010dependent glucose transporter 1, facilitative glucose transporters 5, and facilitative glucose transporters 2 was assessed by RT\u2010PCR. It was found that facilitative glucose transporters 2 expression was increased (60%) in Caco\u20102 cells pretreated with anthocyanins, by comparison with controls. When the effect of anthocyanin extract on<jats:sup>3<\/jats:sup>H\u20102\u2010deoxy\u2010<jats:sc>D<\/jats:sc>\u2010glucose uptake was tested, an inhibitory effect was observed (about 60% decrease). However, the malvidin aglycone was tested and had no effect. In conclusion, anthocyanins could be absorbed through Caco\u20102 cells, and can interfere with their own transport and also with glucose intestinal uptake.<\/jats:p>","DOI":"10.1002\/mnfr.200900007","type":"journal-article","created":{"date-parts":[[2009,9,25]],"date-time":"2009-09-25T18:12:53Z","timestamp":1253902373000},"page":"1430-1437","source":"Crossref","is-referenced-by-count":148,"title":["Absorption of anthocyanins through intestinal epithelial cells \u2013 Putative involvement of GLUT2"],"prefix":"10.1002","volume":"53","author":[{"given":"Ana","family":"Faria","sequence":"first","affiliation":[]},{"given":"Diogo","family":"Pestana","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"F\u00e1tima","family":"Martel","sequence":"additional","affiliation":[]},{"given":"Victor","family":"de Freitas","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Mateus","sequence":"additional","affiliation":[]},{"given":"Concei\u00e7\u00e3o","family":"Calhau","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,11,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715760600758522"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf060300l"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1063::AID-JSFA605>3.0.CO;2-Q"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0308-8146(92)90223-O"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(03)00438-2"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200700092"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.12.4178"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.9.2275"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.7.1865"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/73.5.920"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf020690l"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2003.tb05697.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002100000223"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0531959"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvn054"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06765.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00899.x"},{"key":"e_1_2_6_19_2","first-page":"14","article-title":"The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts","volume":"4","author":"Berridge M. 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