{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T01:52:15Z","timestamp":1772848335868,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,4,17]],"date-time":"2012-04-17T00:00:00Z","timestamp":1334620800000},"content-version":"vor","delay-in-days":76,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>Scope:<\/jats:bold>The aim of this study was to investigate the antihypertensive effect of a peptide fraction (PepC) obtained from a whey protein concentrate following hydrolysis by<jats:italic>Cynara cardunculus<\/jats:italic>, as well as of its fraction with MW below 3 kDa (PepCF). Both these concentrates encompassed peptides that exhibited potent in vitro inhibition of angiotensin\u2010converting enzyme (ACE): two were released from \u03b1\u2010lactalbumin \u2013 KGYGGVSLPEW and DKVGINYW, and one from \u03b2\u2010lactoglobulin \u2013 DAQSAPLRVY.<\/jats:p><jats:p><jats:bold>Methods and results:<\/jats:bold>Upon oral administration, by gastric intubation, of 400 mg\/kg body weight (bw) of those peptide concentrates, or 5 mg\/kg bw of the corresponding synthetic peptides, to 12 wk\u2010old spontaneously hypertensive rats (SHR), the systolic and diastolic blood pressures were monitored by the tail\u2010cuff method \u2013 before, and 2, 4, 6, 8 and 24 h afterwards. Water and zofenopril (5 mg\/kg bw) \u2013 a known ACE\u2010inhibitor, were used as negative and positive controls, respectively. Acute administration of PepC, PepCF, KGYGGVSLPEW, DKVGINYW and DAQSAPLRVY caused antihypertensive effects in SHR; the maximum effect occurred by 4 h and 6 h after administration of the peptide concentrates and the synthetic peptides, respectively. PepC and KGYGGVSLPEW also showed ACE\u2010inhibitory activity in vivo: the pressor effect of angiotensin I was significantly lower, and the response to bradykinin increased when the rats were pre\u2010treated with either product.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold>Our results strongly suggest that PepC will be effective as nutraceutical ingredient for the formulation of functional foods aimed at hypertension control.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201100381","type":"journal-article","created":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T13:19:01Z","timestamp":1323263941000},"page":"316-324","source":"Crossref","is-referenced-by-count":53,"title":["Acute effect of whey peptides upon blood pressure of hypertensive rats, and relationship with their angiotensin\u2010converting enzyme inhibitory activity"],"prefix":"10.1002","volume":"56","author":[{"given":"T\u00e2nia","family":"Tavares","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa Oeiras Portugal"}]},{"given":"Mar\u00eda\u2010\u00c1ngeles","family":"Sevilla","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Farmacolog\u00eda Facultad de Farmacia Universidad de Salamanca Salamanca Spain"}]},{"given":"Mar\u00eda\u2010Jos\u00e9","family":"Montero","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Farmacolog\u00eda Facultad de Farmacia Universidad de Salamanca Salamanca Spain"}]},{"given":"Rosal\u00eda","family":"Carr\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Farmacolog\u00eda Facultad de Farmacia Universidad de Salamanca Salamanca Spain"}]},{"given":"F. 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