{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:47:24Z","timestamp":1777891644847,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T00:00:00Z","timestamp":1397692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PEstOE\/EQB\/LA0004\/2011"],"award-info":[{"award-number":["PEstOE\/EQB\/LA0004\/2011"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/63615\/2009"],"award-info":[{"award-number":["SFRH\/BD\/63615\/2009"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SRFH\/BPD\/84618\/2012"],"award-info":[{"award-number":["SRFH\/BPD\/84618\/2012"]}]},{"name":"Scottish Government Rural and Environment Science and Analytical Services Division"},{"name":"Climafruit (Interreg IVB)","award":["EU FP7 EUBerry KBBE-2010-4 265942"],"award-info":[{"award-number":["EU FP7 EUBerry KBBE-2010-4 265942"]}]},{"name":"European Research Council","award":["POLYTRUE? 322467 to G.W."],"award-info":[{"award-number":["POLYTRUE? 322467 to G.W."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>The colonic metabolism of dietary flavonoids, phenolic acids and their phenolic metabolites is complex and many metabolites and conjugates have not yet been unambiguously identified in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Urine samples from nine healthy human volunteers obtained after the ingestion of a puree of five (poly)phenol\u2010rich berry fruits were analysed using LC\u2010Orbitrap MS to provide a preliminary indication of possible metabolites based on exact mass. In most cases, the identity of compounds was confirmed using standards produced either chemically or enzymically followed by analysis using LC\u2010triple quadrupole MS. Sulphated, glucuronidated and methylated forms of catechol, pyrogallol and protocatechuic acid mostly appeared in urine after 8 h, suggesting colonic metabolism. Gallic acid and (\u2212)\u2010epicatechin conjugates appeared mainly before 4 h, indicative of absorption from the small intestine. Conjugates of ferulic, caffeic, and vanillic acid appeared at intermediate times.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We have positively identified metabolites and conjugates, some novel, in the urine of healthy volunteers after intake of multiple phenolics from a mixed puree from berry fruits, with each being excreted at specific and signature times. Some of these compounds could potentially be used as biomarkers of fruit intake. The possible biological activities of these colonic metabolites require further assessment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201300822","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T07:04:46Z","timestamp":1397718286000},"page":"1414-1425","source":"Crossref","is-referenced-by-count":74,"title":["Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers"],"prefix":"10.1002","volume":"58","author":[{"given":"Rui C.","family":"Pimp\u00e3o","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa Av. da Rep\u00fablica EAN  Oeiras Portugal"}]},{"given":"Tristan","family":"Dew","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition University of Leeds  Leeds UK"}]},{"given":"Maria E.","family":"Figueira","sequence":"additional","affiliation":[{"name":"iMed\u2010UL and Faculdade de Farm\u00e1cia Universidade de Lisboa Av. Prof. Gama Pinto  Lisboa Portugal"}]},{"given":"Gordon J.","family":"McDougall","sequence":"additional","affiliation":[{"name":"The James Hutton Institute  Scotland UK"}]},{"given":"Derek","family":"Stewart","sequence":"additional","affiliation":[{"name":"The James Hutton Institute  Scotland UK"},{"name":"Bioforsk \u2013 Norwegian Institute for Agricultural and Environmental Research  Tromso Norway"}]},{"given":"Ricardo B.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa Av. da Rep\u00fablica EAN  Oeiras Portugal"},{"name":"Instituto Superior de Agronomia Universidade de Lisboa Tapada da Ajuda  Lisboa Portugal"}]},{"given":"Claudia N.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa Av. da Rep\u00fablica EAN  Oeiras Portugal"},{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica  Oeiras Portugal"}]},{"given":"Gary","family":"Williamson","sequence":"additional","affiliation":[{"name":"School of Food Science and Nutrition University of Leeds  Leeds UK"}]}],"member":"311","published-online":{"date-parts":[[2014,4,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2010.00273.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510003910"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2006.03.045"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130-010-0177-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4581"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510003946"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf305498j"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-008-0821-2"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0ob00137f"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/09637486.2013.816938"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01211-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.4.1207"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/np0000421"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200900611"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf402394c"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf3002153"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2FO30151B"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf062230o"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf902263n"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf902267v"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.1885"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-004-0502-2"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf8035116"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201000355"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.049247"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200800461"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac3017339"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.109.028019"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-013-0512-z"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2010.03.005"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.05.023"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100566"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1970.218.5.1307"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.08.014"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac300829f"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf048439o"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf061016x"}],"container-title":["Molecular Nutrition &amp; 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