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Erucin can be generated by in vivo reduction of sulforaphane or by enzymatic hydrolysis of glucoerucin. Contrarily to sulforaphane, only limited studies have addressed the anticancer properties of erucin. This study aims at evaluating the impact of erucin on renal cell biology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>The effects of erucin were assessed in 786\u2010O and Vero\u2010E6 cells, representative of human renal cancer and non\u2010 cancer kidney cells, respectively. Erucin induced a concentration\u2010dependent decrease in cell viability and cell cycle arrest at G2\/Mitosis. In Vero\u2010E6 cells erucin modestly reduced intracellular reactive oxygen species levels while in 786\u2010O no effects were detected. After erucin treatment, both cell lines revealed altered morphology, with a concentration\u2010dependent change from an elongated shape towards a smaller round conformation. Moreover, erucin affected cell adhesion and strongly altered the tubulin network structure and specifically microtubule polymerization. These results are in line with the observed decrease in collective and single cell migration and G2\/Mitosis arrest.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overall, erucin may have a beneficial impact in reducing the motility of renal cancer cells. Our results contribute to explore possible dietary approaches for secondary\/tertiary renal cancer chemoprevention.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200581","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T06:11:07Z","timestamp":1669183867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Dietary Isothiocyanate Erucin Reduces Kidney Cell Motility by Disturbing Tubulin Polymerization"],"prefix":"10.1002","volume":"67","author":[{"given":"\u00cdris","family":"Guerreiro","sequence":"first","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"},{"name":"Facultad de Medicina y Ciencias de la Salud Universidad de Alcal\u00e1  Alcal\u00e1 de Henares 28805 Spain"}]},{"given":"Bojana","family":"Vidovic","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy University of Belgrade  Belgrade 11221 Serbia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5604-6142","authenticated-orcid":false,"given":"Jo\u00e3o G.","family":"Costa","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"}]},{"given":"Marta","family":"Martins","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"},{"name":"Facultad de Medicina y Ciencias de la Salud Universidad de Alcal\u00e1  Alcal\u00e1 de Henares 28805 Spain"}]},{"given":"Sandra","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"},{"name":"Facultad de Medicina y Ciencias de la Salud Universidad de Alcal\u00e1  Alcal\u00e1 de Henares 28805 Spain"}]},{"given":"Nuno G.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa) Faculty of Pharmacy Universidade de Lisboa  Lisbon 1649\u2010003 Portugal"}]},{"given":"Nuno","family":"Saraiva","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6350-0641","authenticated-orcid":false,"given":"Ana S.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon 1749\u2010024 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201800079"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2007.01.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2011.08.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.767975"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2040593"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.03.024"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100599"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2017.04.014"},{"key":"e_1_2_9_9_1","first-page":"845","volume":"33","author":"Citi V.","year":"2019","journal-title":"J. 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