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The resident organisms typical of a healthy gut are well adapted to the gastrointestinal environment while alteration of these populations can trigger disorders that may affect the health and well\u2010being of the host. Various investigations have applied different tools to study bacterial communities in the gut and their correlation with gastrointestinal disorders such as inflammatory bowel disease (IBD), obesity, and diabetes. This study proposes fluorescent in situ hybridization, combined with flow cytometry (FISH\u2010FLOW), as an alternative approach for phylum level identification of Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria and quantification of target bacteria from the GIT based on analysis of fecal samples, where results are validated by quantitative polymerase chain reaction (qPCR) and 16S ribosomal ribonucleic acid (16s rRNA) sequencing. The results obtained via FISH\u2010FLOW experimental approach show high specificity for the developed probes for hybridization with the target bacteria. The study, therefore, suggests the FISH\u2010FLOW as a reliable method for studying bacterial communities in the gut with results correlating well with those of metagenomic investigations of the same fecal samples.<\/jats:p>","DOI":"10.1002\/mnfr.202400494","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T06:50:58Z","timestamp":1728024658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Use of FISH\u2010FLOW as a Method for the Identification and Quantification of Bacterial Populations"],"prefix":"10.1002","volume":"69","author":[{"given":"Jorge Enrique","family":"Vazquez Bucheli","sequence":"first","affiliation":[{"name":"Human Effective Microbes, Department of Advanced Convergence Handong Global University  Pohang Gyeongbuk 37554 Republic of Korea"},{"name":"Facultad de medicina veterinaria y zootecnia Universidad Aut\u00f3noma de Chiapas  Chiapas, Calz. 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