{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:11:50Z","timestamp":1760969510624,"version":"build-2065373602"},"reference-count":61,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":4152,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Reson in Chemistry"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>1D and 2D <jats:sup>1<\/jats:sup>H NMR studies are reported on the oxidized and reduced [4Fe\u20104S] cluster of <jats:italic>Desulfovibrio gigas<\/jats:italic> ferredoxin I (Fdl). Several low\u2010field contact shifted resonances (fast relaxing) are assigned to \u03b2\u2010CH<jats:sub>2<\/jats:sub> and \u03b1\u2010CH coordinated cysteinyl residues. NOESY patterns (supported by 1D NOE experiments) resolves four pairs of geminal \u03b2\u2010CH<jats:sub>2<\/jats:sub> protons at low\u2010field. The cluster ligands are assigned non\u2010specifically to Cys8, Cys11, Cys14 and Cys50, based on the X\u2010ray structural analysis available for the oligomeric form, FdII, that contains a single [3Fe\u20104S] cluster. It was indicated in this case that Cys11 is not bound to the trinuclear cluster but is tilted towards the solvent. The presence of four pairs of geminal \u03b2\u2010CH<jats:sub>2<\/jats:sub> protons for FdI unambiguously proves the occupancy of the fourth site of the [3Fe\u20104S] complex and implies the coordination of the Cys11 at the cluster. Analysis of the oxidized form of FdII, using the same methodology as described for FdI, supports the presence of three cysteinyl ligands in the [3Fe\u20104S] core. Further, the combined use of the X\u2010ray coordinates enables the specific assignment of the three cysteinyl ligands of the cluster, extending a previous assignment of Cys50. In addition, very broad resonances were detected for the reduced form of FdII in the low\u2010field region around 200 ppm and in the high field region around \u221280 ppm.<\/jats:p>","DOI":"10.1002\/mrc.1260311313","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T19:08:38Z","timestamp":1117307318000},"source":"Crossref","is-referenced-by-count":30,"title":["Two\u2010dimensional <sup>1<\/sup>H NMR studies on <i>Desulfovibrio gigas<\/i> ferredoxins. 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