{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T11:38:54Z","timestamp":1774265934741,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3536,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Temporal and spatial characteristics of explosive periodic increases (spikes) of intracellular free Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]i) induced by sperm in human oocytes (Ca<jats:sup>2+<\/jats:sup> oscillations) were analyzed by confocal laser scanning microscopy and compared to Ca<jats:sup>2+<\/jats:sup> oscillations induced in oocytes by the thiol reagent thimerosal. During the steady\u2010state period of sperm\u2010induced Ca<jats:sup>2+<\/jats:sup> oscillations, each individual [Ca<jats:sup>2+<\/jats:sup>]i spike invariably began from a focus in oocyte periphery and spread throughout the entire peripheral region before propagating to the central ooplasm. This peripheral Ca<jats:sup>2+<\/jats:sup> wave was immediately followed by an explosive [Ca<jats:sup>2+<\/jats:sup>]i increase in the central ooplasm. However, this central [Ca<jats:sup>2+<\/jats:sup>]i rise only peaked when [Ca<jats:sup>2+<\/jats:sup>]i in the peripheral ooplasm was already on the decline. Moreover, the peak [Ca<jats:sup>2+<\/jats:sup>]i values were always considerably higher in the oocyte center than in the periphery. In contrast, thimerosal\u2010induced Ca<jats:sup>2+<\/jats:sup> oscillations did not show this particular form of propagation. These data show that sperm\u2010induced Ca<jats:sup>2+<\/jats:sup> oscillations have a unique pattern of spatial dynamics and suggest that the bulk of Ca<jats:sup>2+<\/jats:sup> mobilized during each spike is released from stores that have a relatively high threshold for Ca<jats:sup>2+<\/jats:sup>\u2010induced Ca<jats:sup>2+<\/jats:sup> release (CICR). These stores are poorly developed, if not absent, in the oocyte cortex, and CICR from them is triggered by previous CICR from another type of store with a lower threshold that are preferentially located in the oocyte cortex and act as a detonator. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.1080410217","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T23:06:40Z","timestamp":1109200000000},"page":"259-263","source":"Crossref","is-referenced-by-count":34,"title":["Sperm\u2010induced calcium oscillations of human oocytes show distinct features in oocyte center and 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