{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T06:29:34Z","timestamp":1723876174276},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":8084,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gamete Research"],"published-print":{"date-parts":[[1983,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The spermatozoon of Chiton marginatus is a long uniflagellate cell displaying structural features of \u201cmodified sperm.\u201d The nucleus presents a conical shape with a long apical cylindrical extension. The chromatin is homogeneously dense. Scattered inside the condensed nucleus, a few nuclear lacunae are visible. The acrosomal complex is lacking. Some mitochondria are located in a laterofrontal structure side by side with the nucleus. The typical midpiece is absent. The cytoplasm forms a thin layer around the nucleus and the mitochondria. The proximal centriole is in a basal nuclear indent. The distal centriole serves to form the axoneme tail with the usual microtubular pattern.<\/jats:p><jats:p>During nuclear maturation, the early spermatid nucleus is spherical and contains fine granular chromatin patches. The nuclear envelope shows a deposit of dense material at the base of the nucleus, forming a semicircular invagination occupied by a flocculent mass. In middle spermatid stage, the chromatin gets organized in filaments, coiled as a hank, attached over the inner surface of the basal thickening of the nuclear envelope. The nucleus starts to elongate anteroposteriorly. At the pointed apical portion of the spermatid, a group of microtubules is observed seeming to impose external pressure to the nucleus giving rise to the long apical nuclear point. The mitochondria have a basal position. Late spermatids have an elongated conical nucleus. The chromatin filaments are further condensed, and lacunae appear inside the nucleus. Some mitochondria migrate to a lateral position.<\/jats:p>","DOI":"10.1002\/mrd.1120080405","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T13:30:09Z","timestamp":1109338209000},"page":"345-355","source":"Crossref","is-referenced-by-count":12,"title":["Fine structure of the spermatozoa of Chiton marginatus (mollusca: Amphineura), with special reference to nucleus maturation"],"prefix":"10.1002","volume":"8","author":[{"given":"F.","family":"Russell\u2010Pinto","sequence":"first","affiliation":[]},{"given":"C.","family":"Azevedo","sequence":"additional","affiliation":[]},{"given":"T.","family":"Barandela","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"47","article-title":"The fine structure of the spermatozoon of Patella lusitanica (Gastropoda: Proscobranchia), with special reference to acrosome formation","volume":"13","author":"Azevedo C","year":"1981","journal-title":"J Submicrosc Cytol"},{"key":"e_1_2_1_3_1","unstructured":"AzevedoC CorralL (1982): Ultrastructural study of spermatozoon and spermiogenesis of Pollicipes cornucopia (Crustacea Cirripedia) with special reference to nucleus maturation.J Submicrosc Cytol14354\u2013654."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-018006-9.50006-0"},{"key":"e_1_2_1_5_1","first-page":"305","volume-title":"The Spermatozoon","author":"Baccetti B","year":"1979"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00331478"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00232770"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(63)90346-X"},{"key":"e_1_2_1_9_1","first-page":"415","volume-title":"Comparative Spermatology","author":"Favard P","year":"1970"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(71)90124-2"},{"key":"e_1_2_1_11_1","first-page":"399","article-title":"Comparative morphological investigation into the spermiogenesis among mollusca","volume":"30","author":"Franz\u00e9n \u00c5","year":"1955","journal-title":"Zool Bidr Uppsala"},{"key":"e_1_2_1_12_1","first-page":"355","article-title":"On spermiogenesis, morphology of the spermatozoon, and biology of fertilization among invertebrates","volume":"31","author":"Franz\u00e9n \u00c5","year":"1956","journal-title":"Zool Bidr Uppsala"},{"key":"e_1_2_1_13_1","first-page":"29","volume-title":"Comparative Spermatology","author":"Franz\u00e9n \u00c5","year":"1970"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(81)80065-2"},{"key":"e_1_2_1_15_1","first-page":"531","volume-title":"Comparative Spermatology","author":"Kessel RG","year":"1970"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(77)90013-2"},{"key":"e_1_2_1_17_1","first-page":"411","article-title":"Organizing process of the temporary acrosome in spermatogenesis of the bivalve Lyonsia ventricosa","volume":"10","author":"Kubo M","year":"1978","journal-title":"J Submicrosc Cytol"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00222065"},{"key":"e_1_2_1_19_1","unstructured":"MatteiX (1969):Contribution \u00e0 l'\u00e9tude de la spermiogen\u00e8sis et des spermatozo\u00efdes de poissons par les m\u00e9thodes de la microscopie \u00e9lectronique. Th\u00e8se Facult\u00e9 des Sciences Universit\u00e9 de Montpellier pp1\u2013148."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.35.1.153"},{"key":"e_1_2_1_21_1","first-page":"498","volume-title":"Moluscos Marinhos e das Aguas Salobras de Portugal","author":"Nobre A","year":"1938"},{"key":"e_1_2_1_22_1","first-page":"956","article-title":"Chiton sperm: No acrosome?","volume":"19","author":"Pearse JS","year":"1979","journal-title":"Am Zool"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(70)90029-8"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.62.3.911"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(76)80025-1"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00362404"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01248560"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(71)80090-4"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1981.00381.x"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(74)80083-3"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(75)90013-1"}],"container-title":["Gamete Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.1120080405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.1120080405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T22:58:50Z","timestamp":1697756330000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.1120080405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,1]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1983,1]]}},"alternative-id":["10.1002\/mrd.1120080405"],"URL":"https:\/\/doi.org\/10.1002\/mrd.1120080405","archive":["Portico"],"relation":{},"ISSN":["0148-7280","1554-3919"],"issn-type":[{"value":"0148-7280","type":"print"},{"value":"1554-3919","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,1]]}}}