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Understanding the impact of graphene\u2010based nanocomposites over bacterial activity is critical for design appropriate technical solutions. In this work, antibacterial activity of monolayer and few\u2010layer graphene is tested against Gram\u2010negative and Gram\u2010positive model bacteria. Such graphene\u2010based nanomaterials present different effects on bacteria viability, depending on concentration and shape. The presence of dopant agents can either increase or revert graphene antibacterial effect. Among other bactericidal mechanisms, we also demonstrate that graphene surface polarity is connected to antibacterial properties and can be easily modified by surface charge transfer doping. 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