{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T04:24:48Z","timestamp":1776745488241,"version":"3.51.2"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"Abstract<\/jats:title>\n Adequate perfusion is critical for maintaining normal brain function and aberrations thereof are hallmarks of many diseases. Pseudo\u2010Continuous Arterial Spin Labeling (pCASL) MRI enables noninvasive quantitative perfusion mapping without contrast agent injection and with a higher signal\u2010to\u2010noise ratio (SNR) than alternative methods. Despite its great potential, pCASL remains challenging, unstable, and relatively low\u2010resolution in rodents \u2013 especially in mice \u2013 thereby limiting the investigation of perfusion properties in many transgenic or other relevant rodent models of disease. Here, we address this gap by developing a novel experimental setup for high\u2010resolution pCASL imaging in mice and combining it with the enhanced SNR of cryogenic probes. We show that our new experimental setup allows for optimal positioning of the carotids within the cryogenic coil, rendering labeling reproducible. With the proposed methodology, we managed to increase the spatial resolution of pCASL perfusion images by a factor of 15 in mice; a factor of 6 in rats is gained compared to the state of the art just by virtue of the cryogenic coil. We also show that the improved pCASL perfusion imaging allows much better delineation of specific brain areas, both in healthy animals as well as in rat and mouse models of stroke. Our results bode well for future high\u2010definition pCASL perfusion imaging in rodents.<\/jats:p>","DOI":"10.1002\/nbm.5288","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:01:23Z","timestamp":1731024083000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High\u2010resolution perfusion imaging in rodents using pCASL at 9.4\u00a0T"],"prefix":"10.1002","volume":"38","author":[{"given":"Sara","family":"Pires Monteiro","sequence":"first","affiliation":[{"name":"Champalimaud Research, Champalimaud Foundation Lisbon Portugal"},{"name":"Institute for Systems and Robotics \u2010 Lisboa and Department of Bioengineering Instituto Superior T\u00e9cnico \u2013 Universidade de Lisboa Lisbon Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2379-0861","authenticated-orcid":false,"given":"Lydiane","family":"Hirschler","sequence":"additional","affiliation":[{"name":"C.J. Gorter MRI Center, Department of Radiology Leiden University Medical Center Leiden Netherlands"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4952-1240","authenticated-orcid":false,"given":"Emmanuel L.","family":"Barbier","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, Inserm, Grenoble Institut Neurosciences, U1216 Grenoble France"}]},{"given":"Patricia","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Institute for Systems and Robotics \u2010 Lisboa and Department of Bioengineering Instituto Superior T\u00e9cnico \u2013 Universidade de Lisboa Lisbon Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6681-5876","authenticated-orcid":false,"given":"Noam","family":"Shemesh","sequence":"additional","affiliation":[{"name":"Champalimaud Research, Champalimaud Foundation Lisbon Portugal"}]}],"member":"311","published-online":{"date-parts":[[2024,11,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3348\/kjr.2014.15.5.554"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910230106"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2016150789"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2014.01.016"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00234\u2010015\u20101571\u2010z"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25197"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17743240"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.27931"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3855"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4105"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.23096"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","unstructured":"MuntingLP DerieppeMP VoortmanLM et al.Multi\u2010scale Assessment of Brain Blood Volume and Perfusion in the APP\/PS1 Mouse Model of Amyloidosis.bioRxiv. Published online July 5 2022.10.1101\/2022.07.01.49829","DOI":"10.1101\/2022.07.01.498298"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4580919"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18756218"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.26767"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.61279"},{"key":"e_1_2_10_18_1","volume-title":"Proceedings of the International Society of Magnetic Resonance in medicine 25th annual meeting, Honolulu, Hawai","author":"Uszynski I","year":"2017"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEUROIMAGE.2023.119870"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10425"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.12.049"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3765"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201012850\u2010x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000923"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21629"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410170513"},{"key":"e_1_2_10_27_1","volume-title":"Paxinos and Franklin's the mouse brain in stereotaxic coordinates","author":"Franklin K","year":"2019"},{"key":"e_1_2_10_28_1","volume-title":"The rat brain in stereotaxic coordinates","author":"Paxinos G","year":"2014"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910400308"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2006.10.020"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1985.9"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2006.01.015"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.029462"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24523"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542\u2010198809000\u201000008"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.00559"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.4103\/2394\u20108108.178544"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000124321.60992.87"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3287\u201009.2009"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhu221"},{"key":"e_1_2_10_41_1","volume-title":"Proceedings of the International Society for Magnetic Resonance in medicine (ISMRM)","author":"Lungu Bai\u00e3o R","year":"2024"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.737525"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25897"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.29783"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.24493"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.20329"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1162\/imag_a_00049"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2023.120118"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.27658"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.media.2015.01.004"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2016.08.016"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.03.060<