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Based on the linkage between salivary proteins and systemic diseases, the aim of this work was the identification of\u00a0saliva proteins using proteomics. Salivary proteins were separated using two\u2010dimensional (2\u2010D) gel electrophoresis over a pH range between 3\u201310, digested, and then analyzed by matrix assisted laser desorption\/ionization\u2010time of flight (MALDI\u2010TOF)\u2010TOF mass spectrometry (MS) and tandem mass spectrometry (MS\/MS). Proteins were identified using automated MS and MS\/MS data acquisition. The resulting data were searched against a protein database using an internal Mascot search routine. Ninety spots give identifications with high statistical reliability. Of the identified proteins, 11 were separated and identified in saliva for the first time using proteomics tools. Moreover, three proteins that have not been previously identified in saliva, PLUNC, cystatin A, and cystatin B were identified.<\/jats:p>","DOI":"10.1002\/pmic.200300638","type":"journal-article","created":{"date-parts":[[2004,3,23]],"date-time":"2004-03-23T08:43:47Z","timestamp":1080031427000},"page":"1109-1115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":241,"title":["Identification of human whole saliva protein components using proteomics"],"prefix":"10.1002","volume":"4","author":[{"given":"Rui","family":"Vitorino","sequence":"first","affiliation":[]},{"given":"Maria Jo\u00e3o C.","family":"Lobo","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio J.","family":"Ferrer\u2010Correira","sequence":"additional","affiliation":[]},{"given":"Joshua R.","family":"Dubin","sequence":"additional","affiliation":[]},{"given":"Kenneth B.","family":"Tomer","sequence":"additional","affiliation":[]},{"given":"Pedro M.","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"Francisco M. 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