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In the present work, a novel method as an alternative to current molecular biology\u2010based detection procedures is established. It solely relies on the\u00a0change of fluorescence decay times of the protein's intrinsic fluorophores tryptophan and tyrosine due to protein\u2010protein interaction. Unlike previously utilized related methods, no labelling of the binding partners is required. This opens the possibility to detect proteins and their natural interactions without perturbation due to chemical alteration. The technique uses immobilization of one of the protein partners onto solid supports, which allows performance of protein binding studies in the microarray format. Fluorescence lifetime experiments of proteins in their different binding states have been applied to protease\/protease\u2010substrate pairs, as well as to the tubulin\/kinesin system. Different binding behavior of proteins in solution towards protein partners immobilized on protein microarrays was detected with regard to binding specificity and protein amount. This label\u2010free method for analyzing protein microarrays offers broad applicability ranging from principal investigations of protein interactions to applications in molecular biology and medicine.<\/jats:p>","DOI":"10.1002\/pmic.200300705","type":"journal-article","created":{"date-parts":[[2004,4,20]],"date-time":"2004-04-20T05:03:41Z","timestamp":1082437421000},"page":"1703-1711","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Readout of protein microarrays using intrinsic time resolved UV fluorescence for label\u2010free detection"],"prefix":"10.1002","volume":"4","author":[{"given":"Hans\u2010Martin","family":"Striebel","sequence":"first","affiliation":[]},{"given":"Peter","family":"Schellenberg","sequence":"additional","affiliation":[]},{"given":"Paulius","family":"Grigaravicius","sequence":"additional","affiliation":[]},{"given":"Karl Otto","family":"Greulich","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,5,25]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00257-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5485.1760"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.2741\/stoll"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200206)2:6<611::AID-PROT611>3.0.CO;2-Y"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(00)82849-4"},{"key":"e_1_2_1_7_2","first-page":"577","volume-title":"Physical Chemistry of Biological Interfaces","author":"Arwin H.","year":"2000"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00634-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.072685299"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(02)00016-7"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200209)2:9<1247::AID-PROT1247>3.0.CO;2-V"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(01)01910-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/14\/1\/319"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00176a011"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-7243(200011\/12)15:6<409::AID-BIO628>3.0.CO;2-6"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/bio.697"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79540-1"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052662999"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5299.530"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-80356-7_3"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.20.10614"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e66"},{"volume-title":"Bioconjugate Techniques","year":"1996","author":"Hermanson G. 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