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Inspection of the two\u2010dimensional gel electrophoresis gels revealed that 1\u00a0h following sudden cell exposure to two different concentrations of phenol, leading to the inhibition of exponential growth (600\u00a0mg\/L) or to growth arrest for, at least, 4\u00a0h before inhibited growth resumption (800\u00a0mg\/L), the amount of 68 proteins was increased while the amount of 13 proteins was reduced. The up\u2010regulated proteins include proteins involved in the: (i) oxidative stress response (AhpC, SodB,Tpx and Dsb); (ii) general stress reponse (UspA, HtpG, GrpE and Tig); (iii) energetic metabolism (AcnB, AtpH, Fpr, AceA, NuoE, and MmsA\u20101); (iv) fatty acid biosynthesis (FabB, AccC\u20101 and FabBx1); (v) inhibition of cell division (MinD); (vi) cell envelope biosynthesis (LpxC, VacJ, and MurA); (vii) transcription regulation (OmpR and Fur); and (viii) transport of small molecules (TolC, BraC, AotJ, AapJ, FbpA and OprQ). Among the down\u2010regulated proteins are those involved in nucleotide biosynthesis (PurM, PurL, PyrH and Dcd) and cell motility (FliC). The information emerging from this genome expression profiling and the detailed investigation of the biological role of candidate genes, as targets of phenol toxicity or as determinants of phenol resistance in <jats:italic>P. putida<\/jats:italic> KT2440, will allow more rationale strategies for developing bacteria with greater solvent tolerance with impact in bioremediation and whole\u2010cell biotransformations in media with organic solvents.<\/jats:p>","DOI":"10.1002\/pmic.200300793","type":"journal-article","created":{"date-parts":[[2004,7,7]],"date-time":"2004-07-07T08:07:20Z","timestamp":1089187640000},"page":"2640-2652","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":211,"title":["Insights into <b><i>Pseudomonas putida<\/i><\/b> KT2440 response to phenol\u2010induced stress by quantitative proteomics"],"prefix":"10.1002","volume":"4","author":[{"given":"Pedro M.","family":"Santos","sequence":"first","affiliation":[]},{"given":"Dirk","family":"Benndorf","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"S\u00e1\u2010Correia","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,8,24]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007920050065"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.161038"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00366.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00367.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00370.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(81)90080-9"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.55.11.2850-2855.1989"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0240893"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00377-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03385.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10628.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.26.120192.001023"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-10-3071"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00302-8"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"5704","DOI":"10.1128\/JB.180.21.5704-5711.1998","volume":"180","author":"Lazar S. 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