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Here, we studied the effects of acetic acid treatment on yeast cells by 2\u2010DE, revealing alterations in the levels of proteins directly or indirectly linked with the target of rapamycin (TOR) pathway: amino\u2010acid biosynthesis, transcription\/translation machinery, carbohydrate metabolism, nucleotide biosynthesis, stress response, protein turnover and cell cycle. The increased levels of proteins involved in amino\u2010acid biosynthesis presented a counteracting response to a severe intracellular amino\u2010acid starvation induced by acetic acid. Deletion of <jats:italic>GCN4<\/jats:italic> and <jats:italic>GCN2<\/jats:italic> encoding key players of general amino\u2010acid control (GAAC) system caused a higher resistance to acetic acid indicating an involvement of Gcn4p\/Gcn2p in the apoptotic signaling. Involvement of the TOR pathway in acetic acid\u2010induced apoptosis was also reflected by the higher survival rates associated to a terminal deoxynucleotidyl transferase\u2010mediated dUTP nick end labeling (TUNEL)\u2010negative phenotype and lower reactive oxygen species levels of \u0394<jats:italic>tor1<\/jats:italic> cells. In addition, deletion mutants for several downstream mediators of the TOR pathway revealed that apoptotic signaling involves the phosphatases Pph21p and Pph22p but not Sit4p. Altogether, our results indicate that GAAC and TOR pathways (Tor1p) are involved in the signaling of acetic acid\u2010induced apoptosis.<\/jats:p>","DOI":"10.1002\/pmic.200700816","type":"journal-article","created":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T07:01:15Z","timestamp":1231484475000},"page":"720-732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Yeast protein expression profile during acetic acid\u2010induced apoptosis indicates causal involvement of the TOR pathway"],"prefix":"10.1002","volume":"9","author":[{"given":"Bruno","family":"Almeida","sequence":"first","affiliation":[]},{"given":"Steffen","family":"Ohlmeier","sequence":"additional","affiliation":[]},{"given":"Agostinho J.","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Frank","family":"Madeo","sequence":"additional","affiliation":[]},{"given":"Cec\u00edlia","family":"Le\u00e3o","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Paula","family":"Ludovico","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/15216540500090553"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.10.012"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.3.729"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02477"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2005.07.006"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402090"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-9-2409"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1247904"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200404138"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e01-12-0161"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310160200"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00188.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2003.tb00143.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2004.03.012"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(01)00014-1"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.3.709"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.2.176"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"18443","DOI":"10.1016\/S0021-9258(19)81378-9","article-title":"The yeast lysyl\u2010tRNA synthetase gene. 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