{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:05:38Z","timestamp":1761512738615,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2009,10,6]],"date-time":"2009-10-06T00:00:00Z","timestamp":1254787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FCT","award":["SFRH\/BD\/28563\/2006"],"award-info":[{"award-number":["SFRH\/BD\/28563\/2006"]}]},{"name":"FCT-FEDER","award":["POCI\/QUI\/55519\/2004"],"award-info":[{"award-number":["POCI\/QUI\/55519\/2004"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report in this work on the robustness of ultrasonic energy as a tool to speed the isotopic labeling of proteins using the <jats:sup>18<\/jats:sup>O\u2010decoupling procedure. The first part of the decoupling procedure, comprising protein denaturation, reduction, alkylation and digestion, is done in 8\u2009min under the effects of an ultrasonic field whilst the second part, the isotopic labeling, was assayed with and without the use of ultrasonic energy. Our results clearly demonstrate that the <jats:sup>18<\/jats:sup>O\u2010isotopic labeling in a decoupling procedure cannot be accelerated using an ultrasonic field.<\/jats:p>","DOI":"10.1002\/pmic.200900197","type":"journal-article","created":{"date-parts":[[2009,10,6]],"date-time":"2009-10-06T09:44:54Z","timestamp":1254822294000},"page":"4974-4977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Can ultrasonic energy efficiently speed <sup>18<\/sup>O\u2010labeling of proteins?"],"prefix":"10.1002","volume":"9","author":[{"given":"Ricardo J.","family":"Carreira","sequence":"first","affiliation":[]},{"given":"Carlos","family":"Lodeiro","sequence":"additional","affiliation":[]},{"given":"Mario S.","family":"Diniz","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Moura","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 L.","family":"Capelo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,10,27]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800421"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20116"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050112v"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.03.013"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060508m"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3214"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.07.048"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr800161x"},{"key":"e_1_2_3_10_2","unstructured":"Data Explorer\u2122Software User Guide Section 3 45\u201347."},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac001404c"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900197","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200900197","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200900197","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:26:11Z","timestamp":1761042371000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200900197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,27]]},"references-count":10,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1002\/pmic.200900197"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200900197","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"type":"print","value":"1615-9853"},{"type":"electronic","value":"1615-9861"}],"subject":[],"published":{"date-parts":[[2009,10,27]]},"assertion":[{"value":"2009-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-07-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-10-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}