{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:22:11Z","timestamp":1773660131450,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:00:00Z","timestamp":1366243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nordforsk and Bruker Daltonics"},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"crossref","award":["09CSA043383PR"],"award-info":[{"award-number":["09CSA043383PR"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"crossref","award":["IN825H"],"award-info":[{"award-number":["IN825H"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agencia de Inova\u00e7\u00e3o Portuguesa"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>In the present work, we report a novel on\u2010target protein cleavage method. The method utilizes ultrasonic energy and allows up to 20 samples to be cleaved in 5 min for protein identification and one sample in 30 s for on\u2010tissue digestion. The standard proteins were spotted on a conductive glass slide in a volume of 0.5 \u03bcL followed by 5 min of ultrasonication after trypsin addition. Controls (5 min, 37\u00b0C no ultrasonication) were also assayed. After trypsin addition, digestion of the tissues was enhanced by 30 s of ultrasonication. The samples were analyzed and compared to those obtained by using conventional 3 h heating proteolysis. The low sample volume needed for the digestion and reduction in sample\u2010handling steps and time are the features that make this method appealing to the many laboratories working with high\u2010throughput sample treatment.<\/jats:p>","DOI":"10.1002\/pmic.201200241","type":"journal-article","created":{"date-parts":[[2013,3,4]],"date-time":"2013-03-04T22:52:27Z","timestamp":1362437547000},"page":"1423-1427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["On\u2010target ultrasonic digestion of proteins"],"prefix":"10.1002","volume":"13","author":[{"given":"Hugo M.","family":"Santos","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Centro de Qu\u00edmica Fina e Biotecnologia (CQFB) REQUIMTE Faculdade de Ci\u00eancias e Tecnologia, (FCT) Universidade Nova de Lisboa, (UNL)  Caparica Portugal"}]},{"given":"Petri","family":"Kouvonen","sequence":"additional","affiliation":[{"name":"Turku Centre for Biotechnology University of Turku and \u00c5bo Akademi University  Turku Finland"},{"name":"Department of Biology Institute of Molecular Systems Biology ETH  Zurich Switzerland"}]},{"given":"Jose\u2010Luis","family":"Capelo","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Centro de Qu\u00edmica Fina e Biotecnologia (CQFB) REQUIMTE Faculdade de Ci\u00eancias e Tecnologia, (FCT) Universidade Nova de Lisboa, (UNL)  Caparica Portugal"}]},{"given":"Garry L.","family":"Corthals","sequence":"additional","affiliation":[{"name":"Turku Centre for Biotechnology University of Turku and \u00c5bo Akademi University  Turku Finland"}]}],"member":"311","published-online":{"date-parts":[[2013,4,18]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.07.034"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac026136s"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr050112v"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr7008077"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800086"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401056"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3715"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac052284h"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800401"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.09.078"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0351264"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.05.039"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200241","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201200241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:31:09Z","timestamp":1761046269000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201200241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,18]]},"references-count":12,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1002\/pmic.201200241"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201200241","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,18]]},"assertion":[{"value":"2012-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}