{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T03:04:26Z","timestamp":1776740666404,"version":"3.51.2"},"reference-count":52,"publisher":"Wiley","issue":"18-19","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Technology Foundation"},{"name":"Applied Science Division"},{"name":"Netherlands Organization for Scientific Research","award":["08053"],"award-info":[{"award-number":["08053"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p><jats:italic>Alicycliphilus denitrificans<\/jats:italic> is a versatile, ubiquitous, facultative anaerobic bacterium. <jats:italic>Alicycliphilus denitrificans<\/jats:italic> strain <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content> can use chlorate, nitrate, and oxygen as electron acceptor for growth. Cells display a prolonged lag\u2010phase when transferred from nitrate to chlorate and vice versa. Furthermore, cells adapted to aerobic growth do not easily use nitrate or chlorate as electron acceptor. We further investigated these responses of strain <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content> by differential proteomics, transcript analysis, and enzyme activity assays. In nitrate\u2010adapted cells transferred to chlorate and vice versa, appropriate electron acceptor reduction pathways need to be activated. In oxygen\u2010adapted cells, adaptation to the use of chlorate or nitrate is likely difficult due to the poorly active nitrate reduction pathway and low active chlorate reduction pathway. We deduce that the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ar\u2010type nitrate reductase of strain <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content> also reduces chlorate, which may result in toxic levels of chlorite if cells are transferred to chlorate. Furthermore, the activities of nitrate reductase and nitrite reductase appear to be not balanced when oxygen\u2010adapted cells are shifted to nitrate as electron acceptor, leading to the production of a toxic amount of nitrite. These data suggest that strain <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content> encounters metabolic challenges in environments with fluctuations in the availability of electron acceptors. All MS data have been deposited in the ProteomeXchange with identifier PXD000258.<\/jats:p>","DOI":"10.1002\/pmic.201200571","type":"journal-article","created":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T09:32:25Z","timestamp":1375349545000},"page":"2886-2894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Metabolic response of <i><scp>A<\/scp>licycliphilus denitrificans<\/i> strain <scp>BC<\/scp> toward electron acceptor variation"],"prefix":"10.1002","volume":"13","author":[{"given":"Margreet J.","family":"Oosterkamp","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  Wageningen The Netherlands"}]},{"given":"Sjef","family":"Boeren","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry Wageningen University  Wageningen The Netherlands"}]},{"given":"Caroline M.","family":"Plugge","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  Wageningen The Netherlands"}]},{"given":"Peter J.","family":"Schaap","sequence":"additional","affiliation":[{"name":"Laboratory of Systems and Synthetic Biology Wageningen University  Wageningen The Netherlands"}]},{"given":"Alfons J. M.","family":"Stams","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  Wageningen The Netherlands"}]}],"member":"311","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2008.07.010"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10889869891214222"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10889869891214231"},{"key":"e_1_2_7_5_1","unstructured":"Korenkov V. Romanenko V. Kuznetsov S. Voronov J. Process for purification of industrial waste waters from perchlorates and chlorate. 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