{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T20:01:03Z","timestamp":1761163263316,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/EBB-BIO\/098983\/2008"],"award-info":[{"award-number":["PTDC\/EBB-BIO\/098983\/2008"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:p>\n                    Identification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (\n                    <jats:italic>Oryza sativa<\/jats:italic>\n                    L. ssp.\n                    <jats:italic>japonica<\/jats:italic>\n                    cv. Nipponbare): a control conventional line (C), an\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    \u2010transformed transgenic line (T\n                    <jats:sub>a<\/jats:sub>\n                    ) and a negative segregant (NS\n                    <jats:sub>b<\/jats:sub>\n                    ). We observed that T\n                    <jats:sub>a<\/jats:sub>\n                    and NS\n                    <jats:sub>b<\/jats:sub>\n                    appeared identical (with only one spot differentially abundant\u2014fold difference \u2265 1.5), contrasting with the control (49 spots with fold difference \u22651.5, in both T\n                    <jats:sub>a<\/jats:sub>\n                    and NS\n                    <jats:sub>b<\/jats:sub>\n                    vs. control). Given that in vitro culture was the only event in common between T\n                    <jats:sub>a<\/jats:sub>\n                    and NS\n                    <jats:sub>b<\/jats:sub>\n                    , we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that T\n                    <jats:sub>a<\/jats:sub>\n                    and NS\n                    <jats:sub>b<\/jats:sub>\n                    lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201400018","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T02:04:32Z","timestamp":1412561072000},"page":"124-134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences"],"prefix":"10.1002","volume":"15","author":[{"given":"C\u00e1tia","family":"Fonseca","sequence":"first","affiliation":[{"name":"National Health Institute  Lisboa Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"S\u00e9bastien","family":"Planchon","sequence":"additional","affiliation":[{"name":"Department of Environment and Agrobiotechnologies (EVA) Centre de Recherche Public Gabriel Lippmann  Belvaux Luxembourg"}]},{"given":"T\u00e2nia","family":"Serra","sequence":"additional","affiliation":[{"name":"IBET  Oeiras Portugal"}]},{"given":"Subhash","family":"Chander","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Nelson J. 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Margarida","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa  Oeiras Portugal"},{"name":"IBET  Oeiras Portugal"}]},{"given":"Rita","family":"Batista","sequence":"additional","affiliation":[{"name":"National Health Institute  Lisboa Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa  Oeiras Portugal"}]}],"member":"311","published-online":{"date-parts":[[2014,12,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2011.2150"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2004.02.019"},{"key":"e_1_2_8_4_1","unstructured":"FAO\/ WHO Safety Aspects of Genetically Modified Foods of Plant Origin: Report of a Joint FAO\/WHO Expert Consultation on Foods Derived from Biotechnology.2000."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03194637"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420049275.ch3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0688-684"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2011.2149"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10535-011-0164-x"},{"key":"e_1_2_8_10_1","unstructured":"S. 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