{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T09:50:34Z","timestamp":1774950634806,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T00:00:00Z","timestamp":1421193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00c3\u00a7\u00c3\u00a3o para a Ci\u00c3\u00aancia e Tecnologia","award":["PTDC\/SAU-NEU\/103728\/2008, PTDC\/NEU-NMC\/0205\/2012, and PEst-C\/SAU\/LA0001\/2013-2014"],"award-info":[{"award-number":["PTDC\/SAU-NEU\/103728\/2008, PTDC\/NEU-NMC\/0205\/2012, and PEst-C\/SAU\/LA0001\/2013-2014"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>The quantification of large proteomes across multiple samples has become the major focus of proteomics. In addition to the advantages of in\u2010gel digestion, the extensive time and sample handling required have precluded the use of this type of method for large quantitative screens. Therefore, an adaptation of the in\u2010gel digestion method, termed short\u2010GeLC, is proposed as a faster and more reproducible sample preparation method for quantitative approaches. The proposed methodology was compared with two well\u2010established procedures for sample preparation, GeLC\u2010MS and the classic liquid digestion followed by LC\u2010MS, using a membrane protein\u2010enriched sample. The results show that the short\u2010GeLC approach substantially reduces the amount of sample handling and the overall time required for analysis compared with the gel\u2010based methods without compromising the overall results at the protein identification level. Furthermore, the short\u2010GeLC approach in combination with the SWATH acquisition method leads to the best quantitative results: more proteins were quantified, and the reproducibility was improved. Finally, this method performed well even on challenging samples enriched in membrane proteins.<\/jats:p>","DOI":"10.1002\/pmic.201400221","type":"journal-article","created":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T01:24:24Z","timestamp":1416792264000},"page":"757-762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Short GeLC\u2010SWATH: A fast and reliable quantitative approach for proteomic screenings"],"prefix":"10.1002","volume":"15","author":[{"given":"Sandra Isabel","family":"Anjo","sequence":"first","affiliation":[{"name":"CNC \u2010 Center for Neuroscience and Cell Biology University of Coimbra  Portugal"},{"name":"Faculty of Sciences and Technology University of Coimbra  Portugal"}]},{"given":"C\u00e1tia","family":"Santa","sequence":"additional","affiliation":[{"name":"CNC \u2010 Center for Neuroscience and Cell Biology University of Coimbra  Portugal"},{"name":"Institute for Interdisciplinary Research University of Coimbra  Portugal"}]},{"given":"Bruno","family":"Manadas","sequence":"additional","affiliation":[{"name":"CNC \u2010 Center for Neuroscience and Cell Biology University of Coimbra  Portugal"},{"name":"Biocant \u2010 Biotechnology Innovation Center  Cantanhede Portugal"}]}],"member":"311","published-online":{"date-parts":[[2015,1,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio736"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.12688\/f11000research.12682-12272.v12682"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.O111.016717"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr301201x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1451-4"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600607"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-148-2_10"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900766r"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070492f"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-179"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2702"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2703"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201400221","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201400221","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201400221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:48:57Z","timestamp":1761061737000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201400221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,14]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1002\/pmic.201400221"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201400221","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,14]]},"assertion":[{"value":"2014-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-11-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}