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In this study, protein markers for differentiating populations of this nematode were identified by studying differences among four selected Iberian and one American population. These populations were compared by quantitative proteomics (iTRAQ). From a total of 2860 proteins identified using the public database from the <jats:italic>B. xylophilus<\/jats:italic> genome project, 216 were unambiguous and significantly differentially regulated in the studied populations. Comparisons of their pairwise ratio were statistically treated and supported in order to convert them into discrete character states, suggesting that 141 proteins were not informative as population specific markers. Application of the Character Compatibility methodology on the remaining 75 proteins (belonging to families with different biological functions) excludes 27 which are incompatible among them. Considering only the compatible proteins, the method selects a subset of 30 specific unique protein markers which allowed the compared classification of the Iberian isolates. This approach makes it easier search for diagnostic tools and phylogenetic inference within species and populations of a pathogen exhibiting a high level of genetic diversity.<\/jats:p>","DOI":"10.1002\/pmic.201500106","type":"journal-article","created":{"date-parts":[[2015,12,31]],"date-time":"2015-12-31T02:20:42Z","timestamp":1451528442000},"page":"1006-1014","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Protein markers of <i>Bursaphelenchus xylophilus<\/i> Steiner &amp; Buhrer, 1934 (Nickle, 1970) populations using quantitative proteomics and character compatibility"],"prefix":"10.1002","volume":"16","author":[{"given":"Sergio","family":"Ciordia","sequence":"first","affiliation":[{"name":"Unidad de Proteomica Centro Nacional de Biotecnolog\u00eda, CSIC  Madrid Spain"}]},{"given":"Lee","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC  Madrid Spain"}]},{"given":"Susana C.","family":"Arcos","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC  Madrid Spain"}]},{"given":"Mar\u00eda Rosa","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC  Madrid Spain"}]},{"given":"Mar\u00eda del Carmen","family":"Mena","sequence":"additional","affiliation":[{"name":"Unidad de Proteomica Centro Nacional de Biotecnolog\u00eda, CSIC  Madrid Spain"}]},{"given":"Paula","family":"Zamora","sequence":"additional","affiliation":[{"name":"Centro de Sanidad Forestal de Calabazanos, Consejer\u00eda de Fomento y Medio Ambiente, Direcci\u00f3n General del Medio Natural, Junta de Castilla y Le\u00f3n Pol\u00edgono Industrial de Villamuriel de Cerrato  Palencia Espa\u00f1a"}]},{"given":"Paulo","family":"Vieira","sequence":"additional","affiliation":[{"name":"NemaLab\/ ICAAM (Instituto de Ci\u00eancias Agr\u00e1rias e Ambientais Mediterr\u00e2nicas) Universidade de \u00c9vora  Herdade da Mitra \u00c9vora Portugal"}]},{"given":"Isabel","family":"Abrantes","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology, Department of Life Sciences University of Coimbra  Cal\u00e7ada Martim de Freitas Coimbra Portugal"}]},{"given":"Manuel","family":"Mota","sequence":"additional","affiliation":[{"name":"NemaLab\/ ICAAM (Instituto de Ci\u00eancias Agr\u00e1rias e Ambientais Mediterr\u00e2nicas) Universidade de \u00c9vora  Herdade da Mitra \u00c9vora Portugal"}]},{"given":"Philippe","family":"Castagnone\u2010Sereno","sequence":"additional","affiliation":[{"name":"INRA UMR1355\/Universit\u00e9 de Nice\u2010SophiaAntipolis\/CNRS UMR7254 Institut Sophia Agrobiotech  Sophia Antipolis France"}]},{"given":"Alfonso","family":"Navas","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC  Madrid Spain"}]}],"member":"311","published-online":{"date-parts":[[2016,2,29]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"375","article-title":"A taxonomic review of the genera of the Aphelenchoidea (Fnchs, 1937) Thorne, 1949 (Nematoda: Tylenchida)","volume":"2","author":"Nickle W. 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