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Envisioning to add new insights on the protease networks involved in the regulation of body fluid peptidomes, the authors apply Proteasix software to predict the proteases involved in the generation of the naturally occurring peptides present in six of the most studied human body fluids. Peptidome data is collected from the databases and from experimental studies. The analysis highlights 132 putative proteases from four families with the predominance of serine proteases and metalloproteases. From these, 49 proteases seem to be common to all fluids and are mostly associated to extracellular matrix organization as well as protein\/peptide hormone processing. Data analysis also emphasizes: i) the similarity between plasma and CSF protease profiles; ii) that saliva and tears share proteases involved in the generation of peptides with antimicrobial activity; iii) that urine is the body fluid with the highest number of unique putative proteases, precluding an easy tracing of proteolytic events in this case. Taken together, the analysis emphasizes the intricate modus operandi of proteases, challenged by the interconnected pathways and amplification cascades in which they are involved.<\/jats:p>","DOI":"10.1002\/pmic.201800187","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T10:59:47Z","timestamp":1531479587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Reviewing Mechanistic Peptidomics in Body Fluids Focusing on Proteases"],"prefix":"10.1002","volume":"18","author":[{"given":"Beatriz","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular Departamento de Cirurgia e Fisiologia Faculdade de Medicina da Universidade do Porto  4200\u2010319 Porto Portugal"}]},{"given":"F\u00e1bio","family":"Trindade","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular Departamento de Cirurgia e Fisiologia Faculdade de Medicina da Universidade do Porto  4200\u2010319 Porto Portugal"},{"name":"Instituto de Biomedicina Department of Medical Sciences University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"Ant\u00f3nio S.","family":"Barros","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular Departamento de Cirurgia e Fisiologia Faculdade de Medicina da Universidade do Porto  4200\u2010319 Porto Portugal"}]},{"given":"Julie","family":"Klein","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Institute of Cardiovascular and Metabolic Disease  Toulouse France"},{"name":"Universit\u00e9 Toulouse III Paul\u2010Sabatier  31330 Toulouse France"}]},{"given":"Francisco","family":"Amado","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Org\u00e2nica, Produtos Naturais e Agroalimentares Department of Chemistry University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Org\u00e2nica, Produtos Naturais e Agroalimentares Department of Chemistry University of Aveiro  3810\u2010193 Aveiro Portugal"}]},{"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular Departamento de Cirurgia e Fisiologia Faculdade de Medicina da Universidade do Porto  4200\u2010319 Porto Portugal"},{"name":"Instituto de Biomedicina Department of Medical Sciences University of Aveiro  3810\u2010193 Aveiro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2018,9,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R800012-MCP200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R800035200"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.03.010"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1111"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj089"},{"key":"e_1_2_6_7_1","first-page":"334","volume":"7","author":"Barrett A. 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