{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:02:54Z","timestamp":1790146974562,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100003135","name":"Minnesota Ovarian Cancer Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100003135","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ascites, the pathological accumulation of fluid in the peritoneal cavity, is common at diagnosis and with recurrence in high\u2010grade serous ovarian carcinoma (HGSOC). Higher volumes of ascites fluid are associated with greater likelihood of recurrent disease and poor prognosis. Due to its availability\u2014volume and frequency\u2014and its reflection of tumors and their microenvironments, ascites is a valuable resource for proteomic biomarker discovery. However, ascites has not been widely studied. Similar to plasma, relatively few proteins comprise the majority of the broad dynamic range of protein mass in ascites fluid, necessitating an enrichment or depletion strategy for proteomic sample preparation. ENRICH\u2010iST and ENRICHplus kits enrich low\u2010abundance proteins using paramagnetic beads. These enrichment strategies have not previously been validated for use with ascites fluid. Our goal was to evaluate the ENRICH\u2010iST and ENRICHplus kits using ascites fluid from HGSOC patients with differing extents of tumor resection during debulking surgery. This Dataset Brief describes the technical performance of these enrichment strategies based on DIA\u2013MS proteome depth, reproducibility of identified proteins, Gene Ontology (GO) term enrichment (Biological Processes, Molecular Functions, and Cellular Components), and Human Protein Atlas\u2010based protein abundance ranking. ENRICHplus outperformed ENRICH\u2010iST by 1.4\u2010fold based on depth of proteome coverage. Relative to the total proteome coverage, ENRICH\u2010iST reduced the proportional contribution of the top 12 high\u2010abundance proteins more effectively than ENRICHplus, thereby increasing the representation of lower\u2010abundance proteins despite identifying fewer overall proteins. Analysis of the differential abundance of the proteins enriched from ascites fluid using these enrichment strategies could greatly facilitate HGSOC biomarker development.<\/jats:p>","DOI":"10.1002\/pmic.70185","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:26:20Z","timestamp":1790144780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Paramagnetic Microbead\u2010Based Biofluid Enrichment Method Enables Proteomic Ovarian Cancer Biomarker Development Using Ascites Fluid"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4170-4579","authenticated-orcid":false,"given":"Kathleen M.","family":"Madden","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7972-2369","authenticated-orcid":false,"given":"Carly A. I.","family":"Twigg","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4382-6501","authenticated-orcid":false,"given":"Ella","family":"Shochatovitz","sequence":"additional","affiliation":[{"name":"University of Minnesota School of Medicine  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin L. M.","family":"Boylan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Kirsch","sequence":"additional","affiliation":[{"name":"Bruker Scientific  Billerica Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Assis","sequence":"additional","affiliation":[{"name":"Bruker Scientific  Billerica Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4672-3450","authenticated-orcid":false,"given":"Amy P. N.","family":"Skubitz","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1679-5453","authenticated-orcid":false,"given":"Stefani N.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrep.2024.101755","article-title":"Ovarian Cancer Ascites Proteomic Profile Reflects Metabolic Changes During Disease Progression","volume":"39","author":"Almeida\u2010Nunes D. L.","year":"2024","journal-title":"Biochemistry and Biophysics Reports"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046858"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u20100875\u2010x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010025\u201002438\u2010z"},{"key":"e_1_2_10_6_1","unstructured":"FDA\u2010NIH Biomarker Working Group. BEST (Biomarkers EndpointS and other Tools) Resource [Internet]. Silver Spring (MD): Food and Drug Administration (US); 2016\u2010. FDA\u2010NIH Biomarker Working Group. 2016 Jan 28 [Updated 2025 Jan 7] https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK338449\/Co\u2010published by National Institutes of Health (US) Bethesda (MD)."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2023.100577"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20156297"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2009.06.003"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr0703223"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800313\u2010MCP200"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5c01103"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2024.101939"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrep.2024.101755","article-title":"Ovarian Cancer Ascites Proteomic Profile Reflects Metabolic Changes During Disease Progression","volume":"39","author":"Almeida\u2010Nunes D. 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