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Other factors can be important in the performance of different\u2010sized AuNPs, including surface area, colloidal stability, and curvature effects. Here, the properties of spherical 20\u00a0nm AuNPs and 35\u00a0nm AuNPs functionalized with a specific thiol\u2010modified oligonucleotide\u2013Au nanoprobes are compared when used on a colorimetric assay for the detection of a single nucleotide polymorphism related to lactose intolerance in humans. Successful functionalization of AuNPs is assessed by UV\u2013vis spectroscopy, agarose gel electrophoresis, dynamic and electrophoretic light scattering, and nanoparticle tracking analysis. Statistical differences between Au\u2010nanoprobe DNA target groups are calculated using analysis of variance and a post hoc Tukey's test. These results show that both 35 and 20\u00a0nm Au nanoprobes have similar detection limits using a 0.15 nmol\u00a0dm<jats:sup>\u22123<\/jats:sup> nanoprobe concentration compared to 2.5 nmol\u00a0dm<jats:sup>\u22123<\/jats:sup>. Interestingly, the use of 35\u00a0nm Au nanoprobes allows a reduction of 80% and 48% in the amount of gold and oligonucleotide used in the assay, respectively.<\/jats:p>","DOI":"10.1002\/ppsc.202200137","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T08:53:43Z","timestamp":1665219223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improved Gold Nanoprobes for Detection of Single Nucleotide Polymorphisms: The Influence of Size"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5429-5874","authenticated-orcid":false,"given":"Maria","family":"Enea","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"},{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit Departamento de Qu\u00edmica Faculdade de Ci\u00eancias e Tecnologia da Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8621-4701","authenticated-orcid":false,"given":"Miguel","family":"Peixoto de Almeida","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias da Universidade do Porto  Porto 4169\u2010007 Portugal"}]},{"given":"Andr\u00e9","family":"Dias","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"},{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit Departamento de Qu\u00edmica Faculdade de Ci\u00eancias e Tecnologia da Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"}]},{"given":"Beatriz","family":"Ferreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias da Universidade do Porto  Porto 4169\u2010007 Portugal"}]},{"given":"Carlos","family":"Bernardes","sequence":"additional","affiliation":[{"name":"STABVIDA Investiga\u00e7\u00e3o e Servi\u00e7os em Ci\u00eancias Biol\u00f3gicas Lda  Madan Parque Caparica 2825\u2010182 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6736-6598","authenticated-orcid":false,"given":"Orfeu","family":"Flores","sequence":"additional","affiliation":[{"name":"STABVIDA Investiga\u00e7\u00e3o e Servi\u00e7os em Ci\u00eancias Biol\u00f3gicas Lda  Madan Parque Caparica 2825\u2010182 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-5696","authenticated-orcid":false,"given":"Eul\u00e1lia","family":"Pereira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV Departamento de Qu\u00edmica e Bioqu\u00edmica Faculdade de Ci\u00eancias da Universidade do Porto  Porto 4169\u2010007 Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-2871","authenticated-orcid":false,"given":"Ricardo","family":"Franco","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB \u2013 Institute for Health and Bioeconomy Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"},{"name":"UCIBIO \u2013 Applied Molecular Biosciences Unit Departamento de Qu\u00edmica Faculdade de Ci\u00eancias e Tecnologia da Universidade NOVA de Lisboa  Caparica 2829\u2010516 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1NR11188D"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2016.11.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppsc.201900281"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppsc.201600405"},{"key":"e_1_2_8_5_1","first-page":"529","volume-title":"Comprehensive Analytical Chemistry","author":"Almeida M. 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