{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T23:17:45Z","timestamp":1767136665790,"version":"build-2238731810"},"reference-count":31,"publisher":"Wiley","issue":"10-11","license":[{"start":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T00:00:00Z","timestamp":1221004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cerebrospinal fluid (CSF) A\u03b21\u201038, A\u03b21\u201040, and A\u03b21\u201042 were comparatively analyzed by amyloid\u2010beta SDS\u2010PAGE with Western immunoblot (A\u03b2\u2010SDS\u2010PAGE\/immunoblot), electrochemiluminescence detection and ELISA (MSD\/ELISA) in patients with Alzheimer's disease (AD,\n                    <jats:italic>n<\/jats:italic>\n                    \u2005=\u200540), frontotemporal dementia (FTD,\n                    <jats:italic>n<\/jats:italic>\n                    \u2005=\u200530), and other dementias (\n                    <jats:italic>n<\/jats:italic>\n                    \u2005=\u200550) and nondemented disease controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2005=\u200530). CSF A\u03b2\u2010peptide concentrations were higher and selective decreases of CSF A\u03b21\u201038 in FTD and A\u03b21\u201042 in AD were more evident as measured after SDS\u2010denaturizing of samples by A\u03b2\u2010SDS\u2010PAGE\/immunoblot. The SDS\u2010accessible pool of CSF A\u03b21\u201038 and A\u03b21\u201042, represented by the individual gain of A\u03b2\u2010peptide yield using A\u03b2\u2010SDS\u2010PAGE\/immunoblot, was reduced in both FTD and AD. Accordingly, biomarker accuracies of A\u03b21\u201038 and A\u03b21\u201042 for detection of FTD and AD, respectively declined as determined by MSD\/ELISA. We conclude that a pool of CSF A\u03b21\u201038 and A\u03b21\u201042, which shows disease\u2010specific reductions in FTD and AD, may be bound to carriers and can be released by SDS. Assessing this SDS\u2010accessible A\u03b2\u2010peptide pool may crucially enhance the accuracy of CSF biomarker tests. Identifying disease\u2010specific binding properties of affected A\u03b2 carriers may elucidate pathogenic aspects and open up a novel field for therapeutic approaches.\n                  <\/jats:p>","DOI":"10.1002\/prca.200800006","type":"journal-article","created":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T09:59:03Z","timestamp":1221040743000},"page":"1548-1556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["CSF amyloid\u2010\u03b2 1\u201038 and 1\u201042 in FTD and AD: Biomarker performance critically depends on the detergent accessible fraction"],"prefix":"10.1002","volume":"2","author":[{"given":"Mirko","family":"Bibl","sequence":"first","affiliation":[]},{"given":"Piotr","family":"Lewczuk","sequence":"additional","affiliation":[]},{"given":"Hermann","family":"Esselmann","sequence":"additional","affiliation":[]},{"given":"Brit","family":"Mollenhauer","sequence":"additional","affiliation":[]},{"given":"Hans\u2010Wolfgang","family":"Klafki","sequence":"additional","affiliation":[]},{"given":"Volker","family":"Welge","sequence":"additional","affiliation":[]},{"given":"Stefanie","family":"Wolf","sequence":"additional","affiliation":[]},{"given":"Claudia","family":"Trenkwalder","sequence":"additional","affiliation":[]},{"given":"Markus","family":"Otto","sequence":"additional","affiliation":[]},{"given":"Johannes","family":"Kornhuber","sequence":"additional","affiliation":[]},{"given":"Jens","family":"Wiltfang","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,10]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"271","article-title":"Quantitative neurohistological features of frontotemporal degeneration","volume":"130","author":"Arnold S. 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