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Identification of specific biomarkers for prediction and monitoring of<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>is needed not only for the adequate screening diagnosis but also to assist the design of interventions to prevent or delay progression of this pathology and its attendant complications. Proteomic methods based on<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>hold special promise for the identification of novel biomarkers that might form the foundation for new clinical tests, but to date, their contribution has been somehow unfruitful. Indeed, from more than 300 proteins found differently modulated in body fluids from diabetic patients, approximately 50 were validated with other approaches like<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>or<jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting and the clinical trials are being initiated to employ biofluids\u2019 proteomics (specifically urinary proteomics) in clinical decision. This review provides an overview of<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\u2010based applications in the identification of potential biomarkers for<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>, emphasizing the methodological challenges involved.<\/jats:p>","DOI":"10.1002\/prca.201200044","type":"journal-article","created":{"date-parts":[[2012,9,20]],"date-time":"2012-09-20T10:47:53Z","timestamp":1348138073000},"page":"447-466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Proteome\u2010base biomarkers in diabetes mellitus: Progress on biofluids' protein profiling using mass spectrometry"],"prefix":"10.1002","volume":"6","author":[{"given":"Ana","family":"Isabel Padr\u00e3o","sequence":"first","affiliation":[{"name":"QOPNA Department of Chemistry University of Aveiro Aveiro Portugal"}]},{"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"QOPNA Department of Chemistry University of Aveiro Aveiro Portugal"}]},{"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[{"name":"QOPNA Department of Chemistry University of Aveiro Aveiro Portugal"}]},{"given":"Francisco","family":"Amado","sequence":"additional","affiliation":[{"name":"QOPNA Department of Chemistry University of Aveiro Aveiro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,10,29]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"WHO Definition diagnosis and classification of diabetes mellitus and its complications.1999. 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