{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:46:27Z","timestamp":1777463187214,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":4566,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1996,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Crystals of the tetraheme cytochrome <jats:italic>c<\/jats:italic><jats:sub>3<\/jats:sub> from sulfate\u2010reducing bacteria <jats:italic>Desulfovibrio gigas<\/jats:italic> (Dg) (MW 13 kDa, 111 residues, four heme groups) were obtained and X\u2010ray diffraction data collected to 1.8 \u00c5 resolution. The structure was solved by the method of molecular replacement and the resulting model refined to a conventional <jats:italic>R<\/jats:italic>\u2010factor of 14.9%. The three\u2010dimensional structure shows many similarities to other known crystal structures of tetraheme <jats:italic>c<\/jats:italic><jats:sub>3<\/jats:sub> cytochromes, but it also shows some remarkable differences. In particular, the location of the aromatic residues around the heme groups, which may play a fundamental role in the electron transfer processes of the molecule, are well conserved in the cases of hemes I, III, and IV. However, heme II has an aromatic environment that is completely different to that found in other related cytochromes <jats:italic>c<\/jats:italic><jats:sub>3<\/jats:sub>. Another unusual feature is the presence of a Ca<jats:sup>2+<\/jats:sup> ion coordinated by oxygen atoms supplied by the protein within a loop near the N\u2010terminus. It is speculated that this loop may be stabilized by the presence of this Ca<jats:sup>2+<\/jats:sup> ion, may contribute to heme\u2010redox perturbation, and might even be involved in the specificity of recognition with its redox partner.<\/jats:p>","DOI":"10.1002\/pro.5560050713","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T04:05:08Z","timestamp":1234238708000},"page":"1342-1354","source":"Crossref","is-referenced-by-count":61,"title":["Cytochrome c<sub>3<\/sub> from <i>Desulfovibrio gigas<\/i>: Crystal structure at 1.8 \u00c5 resolution and evidence for a specific calcium\u2010binding site"],"prefix":"10.1002","volume":"5","author":[{"given":"Pedro M.","family":"Matias","sequence":"first","affiliation":[]},{"given":"Jos\u00e9","family":"Morais","sequence":"additional","affiliation":[]},{"given":"Ricardo","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Maria Arm\u00e9nia","family":"Carrondo","sequence":"additional","affiliation":[]},{"given":"Keith","family":"Wilson","sequence":"additional","affiliation":[]},{"given":"Zbigniew","family":"Dauter","sequence":"additional","affiliation":[]},{"given":"Larry","family":"Sieker","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"107","volume-title":"Crystallographic databases \u2014 Information content, software systems, scientific applications","author":"Abola EE","year":"1987"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(77)80200-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1py00061f"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560040908"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/355472a0"},{"key":"e_1_2_1_7_1","volume-title":"XPLOR: A system for X\u2010ray crystallography and NMR","author":"Br\u00fcnger AT","year":"1992"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(87)80024-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"Collaborative Computational Project Number 4. 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