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These particles were shown to accelerate wound healing, through delivery of regenerative mediators, such as microRNAs. Herein we describe an optimized and upscalable process for the isolation of EV smaller than 200\u2009nm (sEV), secreted by umbilical cord blood mononuclear cells (UCB-MNC) under ischemic conditions and propose quality control thresholds for the isolated vesicles, based on the thorough characterization of their protein, lipid and RNA content. Ultrafiltration and size exclusion chromatography (UF\/SEC) optimized methodology proved superior to traditional ultracentrifugation (UC), regarding production time, standardization, scalability, and vesicle yield. Using UF\/SEC, we were able to recover approximately 400 times more sEV per mL of media than with UC, and upscaling this process further increases EV yield by about 3-fold. UF\/SEC-isolated sEV display many of the sEV\/exosomes classical markers and are enriched in molecules with anti-inflammatory and regenerative capacity, such as hemopexin and miR-150. Accordingly, treatment with sEV promotes angiogenesis and extracellular matrix remodeling, in vitro. In vivo, UCB-MNC-sEV significantly accelerate skin regeneration in a mouse model of delayed wound healing. The proposed isolation protocol constitutes a significant improvement compared to UC, the gold-standard in the field. Isolated sEV maintain their regenerative properties, whereas downstream contaminants are minimized. The use of UF\/SEC allows for the standardization and upscalability required for mass production of sEV to be used in a clinical setting.<\/jats:p>","DOI":"10.1002\/sctm.20-0376","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T10:09:59Z","timestamp":1613297399000},"page":"910-921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use"],"prefix":"10.1093","volume":"10","author":[{"given":"Renato M. S.","family":"Cardoso","sequence":"first","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"}]},{"given":"Silvia C.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"},{"name":"Doctoral Programme in Experimental Biology and Biomedicine (PDBEB) Institute for Interdisciplinary Research (IIIUC), CNC\u2014Center for Neuroscience and Cell Biology, University of Coimbra., Coimbra, Portugal"}]},{"given":"Claudia F.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"}]},{"given":"Filipe V.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"}]},{"given":"Maryse","family":"Romao","sequence":"additional","affiliation":[{"name":"Institut Curie Paris Sciences Lettres Research University, Centre National de la Recherche Scientifique, UMR 144, Paris, France"},{"name":"Cell and Tissue Imaging Core Facility PICT-IBiSA Institut Curie, Paris, France"}]},{"given":"Ermelindo C.","family":"Leal","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neurosciences and Cell Biology University of Coimbra, Coimbra, Portugal"},{"name":"3Is\u2014Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal"}]},{"given":"Patricia C.","family":"Freire","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"}]},{"given":"Ricardo","family":"Neves","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"},{"name":"CNC\u2014Center for Neurosciences and Cell Biology University of Coimbra, Coimbra, Portugal"},{"name":"3Is\u2014Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal"}]},{"given":"Joana","family":"Sim\u00f5es-Correia","sequence":"additional","affiliation":[{"name":"Exogenus Therapeutics, S.A, Biocant Park, Cantanhede, Portugal"},{"name":"CNC\u2014Center for Neurosciences and Cell Biology University of Coimbra, Coimbra, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"issue":"16","key":"2022022810522926800_sct312889-bib-0001","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1007\/s00018-011-0689-3","article-title":"Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles","volume":"68","author":"Gy\u00f6rgy","year":"2011","journal-title":"Cell Mol Life Sci"},{"issue":"4","key":"2022022810522926800_sct312889-bib-0002","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1083\/jcb.201211138","article-title":"Extracellular vesicles: Exosomes, microvesicles, and friends","volume":"200","author":"Raposo","year":"2013","journal-title":"J Cell 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