{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T21:40:10Z","timestamp":1736286010180,"version":"3.32.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,2,22]],"date-time":"2006-02-22T00:00:00Z","timestamp":1140566400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Signal Transduction"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acetylcholine (ACh) facilitates its own release acting at muscarinic M<jats:sub>1<\/jats:sub>receptors on rat motoneurons. While the M<jats:sub>1<\/jats:sub>positive feedback mechanism is operative there is a concomitant suppression of the ability of muscarinic M<jats:sub>2<\/jats:sub>\u2010inhibitory and adenosine A<jats:sub>2A<\/jats:sub>\u2010facilitatory receptors to control [<jats:sup>3<\/jats:sup>H]ACh release. We aimed at investigating whether M<jats:sub>1<\/jats:sub>\u2010occlusion of M<jats:sub>2<\/jats:sub>and A<jats:sub>2A<\/jats:sub>receptors function could result from interplay at second messengers level. Drugs blocking the IP<jats:sub>3<\/jats:sub>pathway, like LiCl and 2\u2010aminoethoxydiphenylborane (2\u2010APB), but not the selective PKC inhibitor, chelerythrine, attenuated M<jats:sub>1<\/jats:sub>facilitation by McN\u2010A\u2010343. PKC activation with phorbol 12\u2010myristate 13\u2010acetate mimicked the ability of McN\u2010A\u2010343 to suppress M<jats:sub>2<\/jats:sub>\u2010inhibition and A<jats:sub>2A<\/jats:sub>\u2010facilitation of [<jats:sup>3<\/jats:sup>H]ACh release caused by oxotremorine and CGS 21680C, respectively. Co\u2010application of chelerythrine together with McN\u2010A\u2010343 restored oxotremorine\u2010inhibition and CGS 21680C\u2010facilitation, but this was not observed when the M<jats:sub>1<\/jats:sub>agonist was applied together with LiCl and 2\u2010APB. McN\u2010A\u2010343 also masked facilitation of [<jats:sup>3<\/jats:sup>H]ACh release caused by stimulators of the cyclic AMP pathway, forskolin and rolipram. Data suggest that M<jats:sub>1<\/jats:sub>\u2010facilitation of ACh release results mainly from activation of the IP<jats:sub>3<\/jats:sub>pathway. This mechanism can be dissociated from the way M<jats:sub>1<\/jats:sub>receptor operates suppression of neuromodulation through M<jats:sub>2<\/jats:sub>\u2010inhibitory and A<jats:sub>2A<\/jats:sub>\u2010facilitatory receptors, which might involve secondary PKC activation.<\/jats:p>","DOI":"10.1002\/sita.200500057","type":"journal-article","created":{"date-parts":[[2006,2,22]],"date-time":"2006-02-22T15:46:43Z","timestamp":1140623203000},"page":"19-31","source":"Crossref","is-referenced-by-count":4,"title":["Dissociation between M<sub>1<\/sub>\u2010facilitation of acetylcholine release and crosstalk with A<sub>2A<\/sub>\u2010 and M<sub>2<\/sub>\u2010receptors on rat motoneurons"],"prefix":"10.1002","volume":"6","author":[{"given":"Laura","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"Paulo","family":"Correia\u2010de\u2010S\u00e1","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,2,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(89)90208-3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2003.02428.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00174861"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.769ad.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.02020.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.6.3910"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0031-6997(24)01362-0","article-title":"International Union of Pharmacology. XVII Classification of muscarinic acetylcholine receptors.","volume":"50","author":"Caulfield M.P.","year":"1999","journal-title":"Pharmacol. 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