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Some kinds of antitumor drugs such as doxorubicin (DOX) mainly act on the nucleic acid causing DNA damage, interfering with transcription, and thereby disrupting or blocking the process of cancer cell replication. Herein, a new nanodrug delivery system, the carbon\u2010based nanomaterials (CBNs)\u2010Pluronic F127\u2010DOX (CPD), is designed by using CBNs as a nanocarrier for DOX. As a result, the tumor growth inhibition rate of CPD group is as high as 79.42\u00a0\u00b1 2.83%, and greatly reduces the side effects. The targeting rate of the CPD group of DOX in the tumor nucleus is 36.78%, and the %ID\/g in tumor tissue is 30.09%. The CPD regulates the expression levels of Caspase\u20103, p53, and Bcl\u20102 genes by increasing intracellular reactive oxygen species (ROS) levels and reducing mitochondrial membrane potential, which indicates that mitochondrial\u2010mediated pathways are involved in apoptosis. The CPD nanodrug delivery system increases the effective accumulation of DOX in tumor cell nuclei and tumor tissues, and generates massive ROS, thereby inhibiting tumor growth in vivo, representing a promising agent for anticancer applications.<\/jats:p>","DOI":"10.1002\/smtd.202100539","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T06:30:48Z","timestamp":1637130648000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["An Efficient Carbon\u2010Based Drug Delivery System for Cancer Therapy through the Nucleus Targeting and Mitochondria Mediated Apoptotic Pathway"],"prefix":"10.1002","volume":"5","author":[{"given":"Junfeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. China"}]},{"given":"Chenchen","family":"Li","sequence":"additional","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. China"}]},{"given":"Qianghua","family":"Xue","sequence":"additional","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. China"}]},{"given":"Xuelian","family":"Yin","sequence":"additional","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. 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China"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. China"},{"name":"3B's Research Group I3Bs \u2013 Research Institute on Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine  4805\u2010017 Guimar\u00e3es Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-9918","authenticated-orcid":false,"given":"Yanli","family":"Wang","sequence":"additional","affiliation":[{"name":"Tumor Precision Targeting Research Center &amp; Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University  Shanghai 200444 P. R. 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