{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:24:28Z","timestamp":1767626668368},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":3284,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Pichia sorbitophila<\/jats:italic> is a halotolerant yeast capable of surviving to extracellular NaCl concentrations up to 4 M in mineral medium when glucose or glycerol are the only carbon and energy sources. Evidence is presented here that glycerol, the main compatible solute this yeast accumulates so as to maintain osmotic balance, is actively co\u2010transported with protons. This transport system was shown to be constitutive, not needing induction by either glycerol or salt, and was not repressible by glucose. In glucose\u2010 or glycerol\u2010grown cells, a simple diffusion was detectable, and iterative calculations were performed to calculate kinetic parameters, in the presence and in the absence of NaCl. At 25\u00b0C, pH 5\u00b70, in glucose\u2010grown cells these were: <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> = 0\u00b781 \u00b1 0\u00b711 m<jats:sc>M<\/jats:sc> and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> = 634\u00b72 \u00b1 164\u00b78 \u03bcmol h<jats:sup>\u22121<\/jats:sup> per g (glycerol); <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> = 1\u00b728 \u00b1 0\u00b760 m<jats:sc>M<\/jats:sc> and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> = 558\u00b76 \u00b7 100\u00b76 \u03bcmol h<jats:sup>\u22121<\/jats:sup> per g (protons). Correspondent stoichiometry was approximately 1, either for these conditions or in the presence of 1 <jats:sc>M<\/jats:sc>\u2010NaCl. An increase in acumulation capacity was evident when different concentrations of NaCl were present. This capacity was shown to be dependent on \u0394pH and membrane potential, consistently with an electrogenic character. We suggest that the main role of this system is in osmoregulation, by keeping glycerol accumulated inside the cells, compensating for leakage, due to its liposoluble character.<\/jats:p>","DOI":"10.1002\/yea.320110203","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:55:07Z","timestamp":1117324507000},"page":"111-119","source":"Crossref","is-referenced-by-count":48,"title":["Characterization of a glycerol\/H<sup>+<\/sup> symport in the halotolerant yeast <i>Pichia sorbitophila<\/i>"],"prefix":"10.1002","volume":"11","author":[{"given":"Fernanda","family":"Lages","sequence":"first","affiliation":[]},{"given":"C\u00e2ndida","family":"Lucas","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00427716"},{"key":"e_1_2_1_3_1","volume-title":"Yeasts: Characteristics and Identification","author":"Barnett J. A.","year":"1990"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60058-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-72-3-589"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1986.tb01839.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"10420","DOI":"10.1016\/S0021-9258(19)68636-9","article-title":"Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae","volume":"256","author":"de la Pe\u00f1a P.","year":"1981","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb19788.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-104-2-343"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)81280-L"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.96.1.110"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(89)90373-8"},{"key":"e_1_2_1_13_1","first-page":"2737","article-title":"Glucose and polyol transport systems in Candida intermedia and their regulation","volume":"133","author":"Loureiro\u2010Dias M. C.","year":"1987","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320060303"},{"key":"e_1_2_1_15_1","first-page":"3049","article-title":"Osmotic adjustment and accumulation of organic solutes in whole cells and protoplasts of Saccharomyces cerevisiae","volume":"134","author":"Meikle A. J.","year":"1988","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00445699"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00270792"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80442-0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(79)90286-4"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(79)55066-6"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(85)90021-2"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.37.111"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(82)90526-0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-5-1047"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00406676"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00166787"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.320110203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.320110203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T18:52:15Z","timestamp":1698259935000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.320110203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,2]]}},"alternative-id":["10.1002\/yea.320110203"],"URL":"https:\/\/doi.org\/10.1002\/yea.320110203","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2]]}}}