{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:08:51Z","timestamp":1759104531070},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":3135,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1995,6,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH) genes from <jats:italic>Kluyveromyces marxianus<\/jats:italic> were identified and characterized. The coding region of two of them (<jats:italic>GAP2<\/jats:italic> and <jats:italic>GAP3<\/jats:italic>) is very similar (99\u00b76% homology). The other gene (<jats:italic>GAP1<\/jats:italic>) is only 86% homologous to <jats:italic>GAP2<\/jats:italic> or <jats:italic>GAP3<\/jats:italic> and is responsible for the expression of Gap1p. This protein is extremely homologous to the <jats:italic>K. marxianus<\/jats:italic> cell wall protein p37, presumably involved in flocculation. However, no leader sequence could be detected in this gene. The identification of the three genes was possible with the use of polymerase chain reaction\u2013single\u2010strand conformation polymorphism (PCR\uf8ffSSCP), as it permits us to overcome the difficulties caused by the high homology amongst the genes. Expression of the GAPDH genes under different carbon sources (glucose or ethanol) was assessed either by Northern blot or reverse transcription\u2013PCR\uf8ffSSCP analysis, revealing that genes <jats:italic>GAP1<\/jats:italic> and <jats:italic>GAP2<\/jats:italic>, but not <jats:italic>GAP3<\/jats:italic>, are transcribed. The results also indicate that the transcription of the gene encoding the cell wall protein p37 (Gap1p) is not dependent on the carbon source, in contrast with the expression of the gene <jats:italic>GAP2<\/jats:italic>, which is affected in cells growing in a glucose\u2010depleted medium.<\/jats:p>","DOI":"10.1002\/yea.320110804","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:59:59Z","timestamp":1117324799000},"page":"725-733","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of the glyceraldehyde\u20103\u2010phosphate dehydrogenase gene family from <i>Kluyveromyces marxianus<\/i>\u2014polymerase chain reaction\u2013single\u2010strand conformation polymorphism as a tool for the study of multigenic families"],"prefix":"10.1002","volume":"11","author":[{"given":"P. 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