{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:26:28Z","timestamp":1762863988651,"version":"3.44.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2001,9]]},"DOI":"10.1006\/abio.2001.5231","type":"journal-article","created":{"date-parts":[[2003,5,19]],"date-time":"2003-05-19T12:51:21Z","timestamp":1053348681000},"page":"29-40","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Standardized Measurements and Differential Spectroscopy in Microplates"],"prefix":"10.1006","volume":"296","author":[{"given":"Georg","family":"Geiger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Bernhagen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Bisswanger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herwig","family":"Brunner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Vitzthum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/abio.2001.5231_RF1","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/0003-2697(82)90608-X","article-title":"A simple, rapid and sensitive method for measuring protein concentration in subcellular membrane fractions prepared by sucrose density ultracentrifugation","volume":"119","author":"Simpson","year":"1982","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0003-2697(89)90188-7","article-title":"Use of a microplate reader in an assay of glutathione reductase using 5,5\u2032-dithiobis(2-nitrobenzoic acid)","volume":"183","author":"Cribb","year":"1989","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/0003-2697(83)90189-6","article-title":"Measurement of DNA with an automatic spectrophotometer","volume":"131","author":"Smeaton","year":"1983","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0003-2697(84)90762-0","article-title":"Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus","volume":"138","author":"Jourdan","year":"1984","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0022-1759(88)90150-0","article-title":"A semi-automated microassay for complement activity","volume":"114","author":"Liu","year":"1988","journal-title":"J. Immunol. Methods"},{"key":"10.1006\/abio.2001.5231_RF6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1006\/abio.1999.4298","article-title":"A quantitative fluorescence-based microplate assay for the determination of double-standed DNA using SYBR green I and a standard UV transilluminator gel imaging system","volume":"276","author":"Vitzthum","year":"1999","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF7","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1006\/abio.1997.2177","article-title":"Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation","volume":"249","author":"Singer","year":"1997","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF8","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/(SICI)1097-4636(199802)39:2<286::AID-JBM16>3.0.CO;2-B","article-title":"False positive results in cytotoxicity testing due to unexpectedly volatile compounds","volume":"39","author":"Ciapetti","year":"1998","journal-title":"J. Biomed. Mater. Res."},{"key":"10.1006\/abio.2001.5231_RF9","unstructured":"Pfeiffer, W. 1997, Vorrichtung zur sterilen Anzucht und Beobachtung von Zellen sowie zur Bestimmung von deren Inhaltsstoffen, Patent DE-19619244A1."},{"key":"10.1006\/abio.2001.5231_RF10","unstructured":"Guhl, T. A, and, Johnson, L. R. 1987, Multiwell Tissue Culture Assembly with Features for Reduced Media Evaporation, U.S. Patent 4657867."},{"key":"10.1006\/abio.2001.5231_RF11","unstructured":"Humphries, G. M. K, and, Kung, V. T. 1988, Hydrophilic Microplates for Vertical Beam Photometry, U.S. Patent 4741619."},{"key":"10.1006\/abio.2001.5231_RF12","unstructured":"Nerbe, F. 1995, Mikrotiterplatte, Patent DE-4405375A1."},{"key":"10.1006\/abio.2001.5231_RF13","unstructured":"Suovaniemi, O. A. 1985, Vorrichtung zur fotometrischen Analyse von fl\u00fcssigen Medien, Patent DE-2451769C2."},{"key":"10.1006\/abio.2001.5231_RF14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1006\/abio.1998.2621","article-title":"Multichannel pipettor performance verified by measuring pathlength of reagent dispensed into a microplate","volume":"258","author":"McGown","year":"1998","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF15","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1432-1033.1981.tb05162.x","article-title":"Dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex from Escherichia coli K12: Comparative characterization of the free and the complex-bound component","volume":"114","author":"Schmincke-Ott","year":"1981","journal-title":"Eur. J. Biochem."},{"key":"10.1006\/abio.2001.5231_RF16","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF17","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0021-9258(18)76122-X","article-title":"I. Spectrophotometric constants for common hemoglobin derivatives in human, dog and rabbitt blood","volume":"98","author":"Drabkin","year":"1932","journal-title":"J. Biol. Chem."},{"key":"10.1006\/abio.2001.5231_RF18","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0006-2944(81)90043-0","article-title":"A micromodification of the Drabkin hemoglobin assay for measuring plasma hemoglobin in the range of 5 to 2000 mg\/dl","volume":"26","author":"Moore","year":"1981","journal-title":"Biochem. Med."},{"key":"10.1006\/abio.2001.5231_RF19","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/0003-9861(69)90340-3","article-title":"Purification and properties of a DPNH-TPNH diaphorase from Clostridium kluyverii","volume":"132","author":"Kaplan","year":"1969","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/abio.2001.5231_RF20","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1006\/abio.1996.0171","article-title":"Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies","volume":"236","author":"Zor","year":"1996","journal-title":"Anal. Biochem."},{"year":"1989","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","key":"10.1006\/abio.2001.5231_RF21"},{"year":"1994","series-title":"Enzymkinetik: Theorie und Methoden","author":"Bisswanger","key":"10.1006\/abio.2001.5231_RF22"},{"key":"10.1006\/abio.2001.5231_RF23","unstructured":"Brunner, H, Bernhagen, J, and, Vitzthum, F. 2000, Method for Differential Spectroscopic Measurements, Patent pending PCT\/EP00\/01121, WO 00\/49 393."},{"key":"10.1006\/abio.2001.5231_RF24","unstructured":"Brunner, H, Bernhagen, J, and, Vitzthum, F. 1999, Verfahren f\u00fcr differenzspektroskopische Messungen, Patent DE-199 06 264.1\u201309."},{"key":"10.1006\/abio.2001.5231_RF25","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1111\/j.1749-6632.1949.tb27297.x","article-title":"Attractions of proteins for small molecules and ions","volume":"51","author":"Scatchard","year":"1949","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1006\/abio.2001.5231_RF26","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/BF01025300","article-title":"Binding of nicotinamide nucleotides to dihydrolipoamide dehydrogenase measured with spin-labeled analogs","volume":"4","author":"Schrenk","year":"1985","journal-title":"J. Protein Chem."},{"key":"10.1006\/abio.2001.5231_RF27","doi-asserted-by":"crossref","first-page":"7726","DOI":"10.1016\/S0021-9258(17)32913-7","article-title":"Spectral evidence for a flavin adduct in a monoalkylated derivative of pig heart lipoamide dehydrogenase","volume":"251","author":"Thorpe","year":"1976","journal-title":"J. Biol. Chem."},{"key":"10.1006\/abio.2001.5231_RF28","doi-asserted-by":"crossref","first-page":"8039","DOI":"10.1016\/S0021-9258(18)83147-7","article-title":"Lipoamide dehydrogenase from Escherichia coli","volume":"264","author":"Sahlmann","year":"1989","journal-title":"J. Biol. Chem."},{"year":"1992","series-title":"Chemistry and Biochemistry in Flavoenzymes","author":"Williams","key":"10.1006\/abio.2001.5231_RF29"},{"key":"10.1006\/abio.2001.5231_RF30","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S1874-6047(08)60241-3","article-title":"Flavin-containing dehydrogenases","volume":"13","author":"Williams","year":"1976","journal-title":"Enzymes"},{"key":"10.1006\/abio.2001.5231_RF31","unstructured":"Hafeman, D. 1996, Determination of Light Absorption Pathlength in a Vertical-Beam Photometer, U. S. Patent 9509459."},{"key":"10.1006\/abio.2001.5231_RF32","unstructured":"Portmann, R, Hirschi, A, and, Wellenreiter, A. 1996, Multi-well Titerplate for Instrumental Analysis, U.S. Patent 5540891."},{"key":"10.1006\/abio.2001.5231_RF33","unstructured":"Curtis, R. H, and, Rundell, A. E. 1994, Pipette Calibration System, U.S. Patent 5298978."},{"key":"10.1006\/abio.2001.5231_RF34","unstructured":"Perlman, D. 1999, Cell Culture Plate with Oxygen and Carbon Dioxide-Permeable Waterproof Sealing Membrane, U.S. Patent 5858770."},{"key":"10.1006\/abio.2001.5231_RF35","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF36","first-page":"358","article-title":"The photometric determination of copper in magnesium and magnesium alloys by means of bicinchoninic acid","volume":"20","author":"Tikhonov","year":"1965","journal-title":"Anal. Chem."},{"key":"10.1006\/abio.2001.5231_RF37","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0003-2697(87)90278-8","article-title":"Assay of reducing sugars in the nanomole range with 2,2\u2032-bicinchoninate","volume":"165","author":"Waffenschmidt","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF38","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measuremt with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"year":"1999","series-title":"Handbook of Fluorescent Probes and Research Chemicals","author":"Haugland","key":"10.1006\/abio.2001.5231_RF39"},{"key":"10.1006\/abio.2001.5231_RF40","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0378-4347(00)00627-7","article-title":"Utilization of the non-covalent fluorescent dye, NanoOrange, as a potential clinical diagnostic tool: Nanomolar human serum albumin quantitation","volume":"754","author":"Harvey","year":"2001","journal-title":"J. Chromatogr. B. Biomed. Sci. Appl."},{"key":"10.1006\/abio.2001.5231_RF41","first-page":"431","article-title":"Eine neue charakteristische Farbreaktion der Thymonucleins\u00e4ure","volume":"204","author":"Dische","year":"1929","journal-title":"Biochem. Z."},{"key":"10.1006\/abio.2001.5231_RF42","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1042\/bj0620315","article-title":"A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid","volume":"62","author":"Burton","year":"1956","journal-title":"Biochem. J."},{"key":"10.1006\/abio.2001.5231_RF43","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/0003-2697(77)90721-7","article-title":"A rapid colorimetric assay for DNA","volume":"81","author":"Setaro","year":"1977","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF44","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0003-2697(80)90432-7","article-title":"A rapid colorimetric assay for the estimation of microgram quantities of DNA","volume":"105","author":"Gold","year":"1980","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5231_RF45","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1515\/bchm2.1942.272.1-2.81","article-title":"Zur photometrischen Bestimmung der Nucleoside und Nucleotide auf Grund der Orcinreaktion","volume":"272","author":"Barrenscheen","year":"1941","journal-title":"Z. Physiol. Chem."},{"key":"10.1006\/abio.2001.5231_RF46","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0021-9258(18)73496-0","article-title":"The identification and estimation of hexoses in polysaccharides and glycoproteins by the carbazole method","volume":"131","author":"Gurin","year":"1939","journal-title":"J. Biol. Chem."},{"key":"10.1006\/abio.2001.5231_RF47","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1128\/jcm.30.11.2847-2851.1992","article-title":"Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction","volume":"30","author":"Loeffelholz","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1006\/abio.2001.5231_RF48","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/BF02907468","article-title":"Ultrafast protein determinations using microwave enhancement","volume":"4","author":"Akins","year":"1995","journal-title":"Mol. Biotechnol."},{"key":"10.1006\/abio.2001.5231_RF49","first-page":"496","article-title":"Measurement of protein in 20 seconds using a microwave BCA assay","volume":"12","author":"Akins","year":"1992","journal-title":"Biotechniques"},{"key":"10.1006\/abio.2001.5231_RF50","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0005-2744(75)90120-5","article-title":"Insulin degradation. XV. Use of different assay methods for the study of mechanism of action of glutathione-insulin transhydrogenase","volume":"397","author":"Chandler","year":"1975","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/abio.2001.5231_RF51","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1016\/S0021-9258(18)50814-0","article-title":"Glutathione-dependent synthesis of deoxyribonucleotides: Characterization of the enzymatic mechanism of Escherichia coli glutaredoxin","volume":"254","author":"Holmgren","year":"1979","journal-title":"J. Biol. Chem."},{"key":"10.1006\/abio.2001.5231_RF52","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0076-6879(73)27024-6","article-title":"Ultraviolet difference spectroscopy\u2014New techniques and applications","volume":"27","author":"Donovan","year":"1973","journal-title":"Methods Enzymol."},{"key":"10.1006\/abio.2001.5231_RF53","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/0003-2697(83)90598-5","article-title":"A simple method for studying the solubilization of polycyclic aromatic hydrocarbons in DNA solutions","volume":"130","author":"Chen","year":"1983","journal-title":"Anal. Biochem."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269701952315?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269701952315?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:13:35Z","timestamp":1757708015000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269701952315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,9]]}},"alternative-id":["S0003269701952315"],"URL":"https:\/\/doi.org\/10.1006\/abio.2001.5231","relation":{},"ISSN":["0003-2697"],"issn-type":[{"type":"print","value":"0003-2697"}],"subject":[],"published":{"date-parts":[[2001,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Standardized Measurements and Differential Spectroscopy in Microplates","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1006\/abio.2001.5231","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2001 Academic Press. All rights reserved.","name":"copyright","label":"Copyright"}]}}