{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:04:16Z","timestamp":1761401056193,"version":"3.44.0"},"reference-count":22,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2001,10]]},"DOI":"10.1006\/abio.2001.5336","type":"journal-article","created":{"date-parts":[[2003,5,19]],"date-time":"2003-05-19T12:51:21Z","timestamp":1053348681000},"page":"105-108","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ratiometric Sensing Using Dual-Frequency Lifetime Discrimination"],"prefix":"10.1006","volume":"297","author":[{"given":"Yordan","family":"Kostov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Harms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Govind","family":"Rao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"year":"1999","series-title":"Principles of Fluorescence Spectroscopy","author":"Lakowicz","key":"10.1006\/abio.2001.5336_RF1"},{"key":"10.1006\/abio.2001.5336_RF2","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1063\/1.1141360","article-title":"A 10 GHz frequency-domain fluorometer","volume":"61","author":"Laczko","year":"1990","journal-title":"Rev. Sci. Instr."},{"key":"10.1006\/abio.2001.5336_RF3","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0005-2736(99)00234-5","article-title":"In vitro characterization of a novel polymeric-based pH-sensitive liposome system","volume":"1463","author":"Zignani","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/abio.2001.5336_RF4","first-page":"76","article-title":"Measurement of individual intracellular pH and membrane potential values in living cells","volume":"3600","author":"Slavik","year":"1999","journal-title":"Proc. SPIE Int. Soc. Opt. Eng."},{"key":"10.1006\/abio.2001.5336_RF5","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0003-2697(90)90425-9","article-title":"Dicarboxy-dichlorofluorescein: A new fluorescent probe for measuring acidic intracellular pH","volume":"187","author":"Nedergaard","year":"1990","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5336_RF6","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/0003-2697(91)90237-N","article-title":"Spectral and photophysical studies of benzo[c]xanthene dyes: dual emission pH sensors","volume":"194","author":"Whitaker","year":"1991","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5336_RF7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0091-679X(08)61715-X","article-title":"Intracellular ionized calcium","volume":"41","author":"June","year":"1994","journal-title":"Methods Cell. Biol."},{"key":"10.1006\/abio.2001.5336_RF8","doi-asserted-by":"crossref","first-page":"C747","DOI":"10.1152\/ajpcell.1999.276.3.C747","article-title":"Synthesis and characterization of dual-wavelength Cl\u2212-sensitive fluorescent indicators for ratio imaging","volume":"276","author":"Jayaraman","year":"1999","journal-title":"Am. J. Physiol."},{"key":"10.1006\/abio.2001.5336_RF9","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1366\/0003702001950238","article-title":"A unique oxygen analyzer combining dual emission probe and low-cost ratiometric fluorimeter","volume":"54","author":"Kostov","year":"2000","journal-title":"Appl. Spectrosc."},{"key":"10.1006\/abio.2001.5336_RF10","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1039\/b002348p","article-title":"Ratiometric oxygen sensing: Detection of dual-emission ratio through a single emission filter","volume":"125","author":"Kostov","year":"2000","journal-title":"Analyst"},{"key":"10.1006\/abio.2001.5336_RF11","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0143-4160(95)90053-5","article-title":"IgG-induced Ca2+ oscillations in differentiated U937 cells: A study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes","volume":"18","author":"Floto","year":"1995","journal-title":"Cell Calcium"},{"key":"10.1006\/abio.2001.5336_RF12","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1021\/ac9914364","article-title":"Dual lifetime referencing as applied to a chloride optical sensor","volume":"73","author":"Huber","year":"2001","journal-title":"Anal. Chem."},{"key":"10.1006\/abio.2001.5336_RF13","doi-asserted-by":"crossref","first-page":"5115","DOI":"10.1021\/ac980876c","article-title":"Low-frequency modulation sensors using nanosecond fluorophores","volume":"70","author":"Lakowicz","year":"1998","journal-title":"Anal. Chem."},{"key":"10.1006\/abio.2001.5336_RF14","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1006\/abio.2000.4486","article-title":"Fluorescence properties of rhodamine 800 in whole blood and plasma","volume":"279","author":"Abugo","year":"2000","journal-title":"Anal. Biochem."},{"key":"10.1006\/abio.2001.5336_RF15","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/nbt0396-315","article-title":"Improved green fluorescent protein by molecular evolution using DNA shuffling","volume":"14","author":"Crameri","year":"1996","journal-title":"Nature Biotechnol."},{"key":"10.1006\/abio.2001.5336_RF16","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/1097-0290(20010205)72:3<346::AID-BIT12>3.0.CO;2-X","article-title":"Low-cost microbioreactor for high throughput bioprocessing","volume":"72","author":"Kostov","year":"2001","journal-title":"Biotechnol. Bioeng."},{"key":"10.1006\/abio.2001.5336_RF17","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/s002160051299","article-title":"Long-lifetime based pH micro-optodes without oxygen interference","volume":"364","author":"Kosch","year":"1999","journal-title":"Fresenius' J. Anal. Chem."},{"year":"1999","series-title":"Optics and Optical Instruments Catalog","key":"10.1006\/abio.2001.5336_RF18"},{"key":"10.1006\/abio.2001.5336_RF19","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1002\/(SICI)1097-0290(19970920)55:6<921::AID-BIT9>3.0.CO;2-I","article-title":"On-line green fluorescent protein sensor with LED excitation","volume":"55","author":"Randers-Eichhorn","year":"1997","journal-title":"Biotechnol. Bioeng."},{"year":"1998","series-title":"GFP Green Fluorescent Protein: Properties, Applications and Protocols","author":"Chalfie","key":"10.1006\/abio.2001.5336_RF20"},{"key":"10.1006\/abio.2001.5336_RF21","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/S0006-3495(98)77870-1","article-title":"Green fluorescent protein as a noninvasive intracellular pH indicator","volume":"74","author":"Kneen","year":"1998","journal-title":"Biophys. J."},{"key":"10.1006\/abio.2001.5336_RF22","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/0143-4160(93)90040-D","article-title":"Ratiometric confocal Ca(2+)-measurements with visible wavelength indicators in isolated cardiac myocytes","volume":"14","author":"Lipp","year":"1993","journal-title":"Cell Calcium"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269701953369?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269701953369?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:12:59Z","timestamp":1757707979000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269701953369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["S0003269701953369"],"URL":"https:\/\/doi.org\/10.1006\/abio.2001.5336","relation":{},"ISSN":["0003-2697"],"issn-type":[{"type":"print","value":"0003-2697"}],"subject":[],"published":{"date-parts":[[2001,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ratiometric Sensing Using Dual-Frequency Lifetime Discrimination","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1006\/abio.2001.5336","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2001 Academic Press. All rights reserved.","name":"copyright","label":"Copyright"}]}}