{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T10:04:44Z","timestamp":1696932284588},"reference-count":21,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2001,8]]},"DOI":"10.1006\/bbrc.2001.5337","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T13:50:03Z","timestamp":1032184203000},"page":"6-11","source":"Crossref","is-referenced-by-count":16,"title":["Rapid Purification of Recombinant Anthrax-Protective Antigen under Nondenaturing Conditions"],"prefix":"10.1006","volume":"286","author":[{"given":"Nidhi","family":"Ahuja","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh","family":"Bhatnagar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/bbrc.2001.5337_RF1","series-title":"Sourcebook of Bacterial Protein Toxins","first-page":"277","article-title":"The anthrax toxin complex","author":"Leppla","year":"1991"},{"key":"10.1006\/bbrc.2001.5337_RF2","first-page":"41","article-title":"A model for anthrax lethal toxin action at the cellular level","volume":"68","author":"Leppla","year":"1990","journal-title":"Salisbury Medical Bulletin"},{"key":"10.1006\/bbrc.2001.5337_RF3","doi-asserted-by":"crossref","first-page":"10277","DOI":"10.1073\/pnas.89.21.10277","article-title":"Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin","volume":"89","author":"Klimpel","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/bbrc.2001.5337_RF4","doi-asserted-by":"crossref","first-page":"20607","DOI":"10.1016\/S0021-9258(17)32036-7","article-title":"Anthrax protective antigen forms oligomers during intoxication of mammalian cells","volume":"269","author":"Milne","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1006\/bbrc.2001.5337_RF5","doi-asserted-by":"crossref","first-page":"19103","DOI":"10.1016\/S0021-9258(19)47273-6","article-title":"A deleted variant of B. anthracis protective antigen is nontoxic and blocks anthrax toxin action in vivo","volume":"264","author":"Singh","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1006\/bbrc.2001.5337_RF6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0898-6568(98)00041-2","article-title":"Activation of phospholipase C and protein kinase C is required for expression of anthrax lethal toxin cytotoxicity in J774A. 1 cells","volume":"11","author":"Bhatnagar","year":"1999","journal-title":"Cell. Signal."},{"key":"10.1006\/bbrc.2001.5337_RF7","doi-asserted-by":"crossref","first-page":"11099","DOI":"10.1016\/S0021-9258(18)60433-8","article-title":"Internalization and processing of Bacillus anthracis lethal toxin-sensitive and resistance cells","volume":"254","author":"Singh","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1006\/bbrc.2001.5337_RF8","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1128\/iai.57.7.2107-2114.1989","article-title":"Calcium is required for expression of anthrax lethal toxin activity","volume":"57","author":"Bhatnagar","year":"1989","journal-title":"Infect. lmmun."},{"key":"10.1006\/bbrc.2001.5337_RF9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1099\/00221287-26-1-49","article-title":"Purification of factor I and recognition of a third factor of anthrax toxin","volume":"26","author":"Stanley","year":"1961","journal-title":"J. Gen. Microbiol."},{"key":"10.1006\/bbrc.2001.5337_RF10","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0168-1656(96)01569-6","article-title":"Thermostabilization of protective antigen\u2013The binding component of anthrax lethal toxin","volume":"50","author":"Radha","year":"1996","journal-title":"J. Biotechnol."},{"key":"10.1006\/bbrc.2001.5337_RF11","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/0092-8674(83)90402-6","article-title":"Cloning of the protective antigen gene of B","volume":"34","author":"Vodkin","year":"1983","journal-title":"anthracis. Cell"},{"key":"10.1006\/bbrc.2001.5337_RF12","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0378-1119(88)90439-8","article-title":"Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis","volume":"69","author":"Welkos","year":"1988","journal-title":"Gene"},{"key":"10.1006\/bbrc.2001.5337_RF13","series-title":"Methods in Enzymology","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0076-6879(88)65019-1","article-title":"Production and purification of anthrax toxin","author":"Leppla","year":"1988"},{"key":"10.1006\/bbrc.2001.5337_RF14","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1042\/bj2520753","article-title":"Purification of anthrax toxin components by high performance anion-exchange, gel filtration, and hydrophobic interaction chromatography","volume":"252","author":"Quinn","year":"1988","journal-title":"Biochem. J."},{"key":"10.1006\/bbrc.2001.5337_RF15","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1128\/IAI.54.2.537-542.1986","article-title":"Cloning and expression of the B. anthracis protective antigen gene in B. subtilis","volume":"54","author":"Ivins","year":"1986","journal-title":"Infect. Immun."},{"key":"10.1006\/bbrc.2001.5337_RF16","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1128\/IAI.58.2.366-372.1990","article-title":"Expression of Bacillus anthracis protective antigen by baculovirus and vaccinia virus recombinants","volume":"58","author":"Iacono-Connors","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1006\/bbrc.2001.5337_RF17","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1006\/prep.1999.1066","article-title":"Expression and purification of the recombinant protective antigen of Bacillus anthracis","volume":"16","author":"Gupta","year":"1999","journal-title":"Prot. Exp. Purif."},{"key":"10.1006\/bbrc.2001.5337_RF18","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1006\/bbrc.2001.4320","article-title":"Trp 346 and Leu 352 residues in protective antigen are required for the expression of anthrax lethal toxin activity","volume":"281","author":"Batra","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1006\/bbrc.2001.5337_RF19","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1006\/prep.1996.0005","article-title":"Expression and purification of anthrax toxin protective antigen from E. coli","volume":"7","author":"Sharma","year":"1996","journal-title":"Prot. Exp. Purif."},{"key":"10.1006\/bbrc.2001.5337_RF20","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","article-title":"Anthrax toxin edema factor: A bacterial adenylate cyclase that increases cAMP concentration in eukaryotic cells","volume":"79","author":"Leppla","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/bbrc.2001.5337_RF21","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1093\/intimm\/12.9.1255","article-title":"Reconstitution of conformationally-dependent epitopes on the N-terminal extracellular domain of the human muscle acetylcholine receptor subunit expressed in Escherichia coli: Implications for myasthenia gravis therapeutic approaches","volume":"12","author":"Tsouloufis","year":"2000","journal-title":"Intern. Immun."}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X01953379?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X01953379?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T22:15:34Z","timestamp":1680560134000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X01953379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["S0006291X01953379"],"URL":"https:\/\/doi.org\/10.1006\/bbrc.2001.5337","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}