{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:16:39Z","timestamp":1760706999441},"reference-count":27,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemical and Biophysical Research Communications"],"published-print":{"date-parts":[[2002,3]]},"DOI":"10.1006\/bbrc.2002.6485","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T18:54:13Z","timestamp":1033930453000},"page":"593-597","source":"Crossref","is-referenced-by-count":16,"title":["Substrate Specificity of Penicillin Acylase from Streptomyces lavendulae"],"prefix":"10.1006","volume":"291","author":[{"given":"Raquel","family":"Torres-Guzm\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"de la Mata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Torres-Bacete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Arroyo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u0131\u0301a Pilar","family":"Castill\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Acebal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/bbrc.2002.6485_RF1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/BF02798404","article-title":"Penicillin acylases: An update","volume":"9","author":"Mahajan","year":"1984","journal-title":"Appl. Biochem. Bioetech."},{"key":"10.1006\/bbrc.2002.6485_RF2","series-title":"Bioreactor, Immobilized Enzymes and Cells; Fundamentals and Applications","first-page":"261","article-title":"Immobilized biocatalyst and antibiotic production: Biochemical, genetic and biotechnical aspects","author":"Vandamme","year":"1988"},{"key":"10.1006\/bbrc.2002.6485_RF3","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/S0141-0229(96)00176-7","article-title":"Penicillin V acylase: Its potential in the production of 6-aminopenicillanic acid","volume":"20","author":"Shewale","year":"1997","journal-title":"Enzyme Microb. Technol."},{"key":"10.1006\/bbrc.2002.6485_RF4","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s002530051618","article-title":"Enhanced production of penicillin V acylase from Streptomyces lavendulae","volume":"53","author":"Torres","year":"1999","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1006\/bbrc.2002.6485_RF5","series-title":"Stability and Stabilization of Biocatalysts","first-page":"719","article-title":"Purification and characterization of penicillin V acylase from Streptomyces lavendulae","author":"Torres","year":"1998"},{"key":"10.1006\/bbrc.2002.6485_RF6","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/S0141-0229(99)00057-5","article-title":"Interaction of penicillin V acylase from with organic solvents: Catalytic activity modulation on the hydrolysis of penicillin V","volume":"25","author":"Arroyo","year":"1999","journal-title":"Enzyme Microb. Technol."},{"key":"10.1006\/bbrc.2002.6485_RF7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/S0141-0229(00)00183-6","article-title":"Prediction of penicillin V acylase stability in water\u2013organic co-solvent monophasic systems as a function of solvent composition","volume":"27","author":"Arroyo","year":"2000","journal-title":"Enzyme Microb. Technol."},{"key":"10.1006\/bbrc.2002.6485_RF8","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1021\/bp000023h","article-title":"Activation and Stabilization of penicillin V acylase from Streptomyces lavendulae in the presence of glycerol and glycols","volume":"16","author":"Arroyo","year":"2000","journal-title":"Biotechnol. Progress."},{"key":"10.1006\/bbrc.2002.6485_RF9","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1023\/A:1005601607277","article-title":"Covalent immobilization of penicillin V acylase from Streptomyces lavendulae","volume":"22","author":"Torres-Bacete","year":"2000","journal-title":"Biotechnol. Lett."},{"key":"10.1006\/bbrc.2002.6485_RF10","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1042\/BA20000042","article-title":"Optimization of 6-APA production by using a new immobilized penicillin acylase","volume":"32","author":"Torres-Bacete","year":"2000","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1006\/bbrc.2002.6485_RF11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF00360873","article-title":"Molecular biology of beta-lactam acylases","volume":"10","author":"Desphande","year":"1994","journal-title":"World. J. Microbial. Technol."},{"key":"10.1006\/bbrc.2002.6485_RF12","first-page":"146","article-title":"Penicillin acylases: Enzyme production and its application in the manufacture of 6-APA","volume":"24","author":"Shewale","year":"1989","journal-title":"Process Biochem."},{"key":"10.1006\/bbrc.2002.6485_RF13","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1128\/AEM.49.5.1084-1089.1985","article-title":"Molecular cloning of Bacillus sphericus penicillin V acylase gene and its expression in E. coli and B. subtilis","volume":"94","author":"Olsson","year":"1985","journal-title":"Appl. Envirom. Microbiol."},{"key":"10.1006\/bbrc.2002.6485_RF14","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/BF00252550","article-title":"Cloning and expression of penicillin acylase genes for overproducing strains of E. coli and Bacillus megaterium","volume":"25","author":"Meevootisom","year":"1987","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1006\/bbrc.2002.6485_RF15","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1006\/abio.1993.1129","article-title":"A colorimetric assay for penicillin V amidase","volume":"209","author":"Kerr","year":"1993","journal-title":"Anal. Biochem."},{"key":"10.1006\/bbrc.2002.6485_RF16","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.2042-7158.1989.tb06412.x","article-title":"Determination of cephalosporin C amidohydrolase activity with fluorescamine","volume":"4","author":"Reyes","year":"1989","journal-title":"J. Pharma. Pharmacol."},{"key":"10.1006\/bbrc.2002.6485_RF17","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1006\/bbrc.2002.6485_RF18","series-title":"Enzyme Kinetics","author":"Segel","year":"1975"},{"key":"10.1006\/bbrc.2002.6485_RF19","first-page":"1","article-title":"The kinetic mechanism of penicillin V acylase from Streptomyces lavendulae","volume":"14","author":"Torres-Guzman","year":"2001","journal-title":"Biocatal. Biotransform."},{"key":"10.1006\/bbrc.2002.6485_RF20","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1002\/bit.260250212","article-title":"Kinetic mechanism of acyl transfer by penicillin acylases","volume":"25","author":"Konecny","year":"1983","journal-title":"Biotechnol. Bioeng."},{"key":"10.1006\/bbrc.2002.6485_RF21","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1042\/bj3160409","article-title":"Rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin G acylase from Kluyvera citrophyla","volume":"316","author":"Roa","year":"1996","journal-title":"Biochem. J."},{"key":"10.1006\/bbrc.2002.6485_RF22","series-title":"Enzymatic Reactions and Mechanisms","author":"Walsh","year":"1979"},{"key":"10.1006\/bbrc.2002.6485_RF23","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1007\/BF00172550","article-title":"Penicillin acylase mutants with altered site-directed activity from Kluyvera citrophyla","volume":"33","author":"Prieto","year":"1990","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1006\/bbrc.2002.6485_RF24","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1006\/jmbi.1998.2180","article-title":"Ligand induced conformational change in penicillin acylase","volume":"284","author":"Done","year":"1998","journal-title":"J. Mol. Biol."},{"key":"10.1006\/bbrc.2002.6485_RF25","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/8213","article-title":"Penicillin vacylase crystal structure reveals new Ntn-hydrolase family members","volume":"6","author":"Suresh","year":"1999","journal-title":"Nature Structural Biology"},{"key":"10.1006\/bbrc.2002.6485_RF26","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1098\/rspb.1961.0050","article-title":"6-aminopenicillanic acid. VI. Formation of 6-aminopenicillanic acid from penicillins by enzymatic hydrolysis","volume":"154","author":"Batchelor","year":"1961","journal-title":"Proc. Roy. Soc. (London) Ser B."},{"key":"10.1006\/bbrc.2002.6485_RF27","series-title":"The Hydrophobic Fragmental Constant","author":"Rekker","year":"1977"}],"container-title":["Biochemical and Biophysical Research Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X02964855?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0006291X02964855?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T13:08:12Z","timestamp":1583413692000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0006291X02964855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["S0006291X02964855"],"URL":"https:\/\/doi.org\/10.1006\/bbrc.2002.6485","relation":{},"ISSN":["0006-291X"],"issn-type":[{"value":"0006-291X","type":"print"}],"subject":[],"published":{"date-parts":[[2002,3]]}}}