{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:10:39Z","timestamp":1791843039783,"version":"4.3.4"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biologicals"],"published-print":{"date-parts":[[2000,6]]},"DOI":"10.1006\/biol.2000.0249","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T10:51:07Z","timestamp":1032173467000},"page":"119-127","source":"Crossref","is-referenced-by-count":38,"title":["In Vitro Markers and Biological Activity in Mice of Seed Lot Strains and Commercial Brucella melitensis Rev 1 and Brucella abortus B19 Vaccines"],"prefix":"10.1006","volume":"28","author":[{"given":"M.J.","family":"Grill\u00f3","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Bosseray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Blasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1006\/biol.2000.0249_RF1","unstructured":"Rodr\u0131\u0301guez Torres, A, Abad, R, Brucelosis humana, 23, 25, Noviembre 1994, Madrid"},{"key":"10.1006\/biol.2000.0249_RF2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0167-5877(96)01110-5","article-title":"A review of the use of Brucella melitensis Rev 1 vaccine in adult sheep and goats","volume":"31","author":"Blasco","year":"1997","journal-title":"Prev Vet Med"},{"key":"10.1006\/biol.2000.0249_RF3","first-page":"793","article-title":"Rev 1 Brucella melitensis vaccine. A review of ten years of study","volume":"37","author":"Alton","year":"1967","journal-title":"Vet Bull"},{"key":"10.1006\/biol.2000.0249_RF4","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0021-9975(67)90038-2","article-title":"Rev 1 Brucella melitensis vaccine. The stability of the degree of attenuation","volume":"77","author":"Alton","year":"1967","journal-title":"J Comp Path"},{"key":"10.1006\/biol.2000.0249_RF5","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/j.1751-0813.1980.tb09561.x","article-title":"Vaccination of pregnant cows with low doses of Brucella abortus strain 19 vaccine","volume":"56","author":"Alton","year":"1980","journal-title":"Aust Vet J"},{"key":"10.1006\/biol.2000.0249_RF6","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/0007-1935(85)90046-6","article-title":"The effect of reduced dose Brucella abortus strain 19 vaccination in accredited dairy herds","volume":"141","author":"Beckett","year":"1985","journal-title":"B Vet J"},{"key":"10.1006\/biol.2000.0249_RF7","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/S0034-5288(18)32468-8","article-title":"Persistence of Brucella abortus strain 19 infection in adult cattle vaccinated with reduced doses","volume":"31","author":"Corner","year":"1981","journal-title":"Res Vet Scien"},{"key":"10.1006\/biol.2000.0249_RF8","first-page":"67","article-title":"Rev 1 Brucella melitensis vaccine. Part II. 1968\u20131980","volume":"2","author":"Elberg","year":"1981","journal-title":"Vet Bull"},{"key":"10.1006\/biol.2000.0249_RF9","first-page":"1193","article-title":"Rev 1 Brucella melitensis vaccine. Part III. 1981\u20131995","volume":"66","author":"Elberg","year":"1996","journal-title":"Vet Bull"},{"key":"10.1006\/biol.2000.0249_RF10","first-page":"143","article-title":"Prevalence and persistence of Brucella abortus strain 19 infections and prevalence of other biotypes in vaccinated adult dairy cattle","volume":"178","author":"Nicoletti","year":"1981","journal-title":"Am J Vet Res"},{"key":"10.1006\/biol.2000.0249_RF11","series-title":"Round Table on the use of Rev 1 vaccine in small ruminants and cattle","first-page":"7","article-title":"Living vaccines: virulence, immunogenicity\/ protection, and safety: Historical, theoretical, and practical considerations applied to the Brucella melitensis Rev 1 vaccine","author":"Plommet","year":"1995"},{"key":"10.1006\/biol.2000.0249_RF12","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S1045-1056(05)80025-9","article-title":"Brucella melitensis Rev 1 attenuated vaccine: Stability of markers, residual virulence and immunogenicity in mice","volume":"19","author":"Bosseray","year":"1991","journal-title":"Biologicals"},{"key":"10.1006\/biol.2000.0249_RF13","series-title":"Brucella melitensis","first-page":"229","article-title":"Quality control of four Rev 1 anti- Brucella vaccines","author":"Bosseray","year":"1985"},{"key":"10.1006\/biol.2000.0249_RF14","first-page":"121","article-title":"Control methods and thresholds of acceptability for anti- Brucella vaccines","volume":"79","author":"Bosseray","year":"1992","journal-title":"Dev Biol Stand"},{"key":"10.1006\/biol.2000.0249_RF15","series-title":"Prevention of Brucellosis in Mediterranean countries","first-page":"182","article-title":"Le vaccin Rev 1: derive des caracteres de immunogenicite et de virulence independante des marqueurs classiques","author":"Bosseray","year":"1992"},{"key":"10.1006\/biol.2000.0249_RF16","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0092-1157(76)80019-4","article-title":"Transformation normalisant la distribution du nombre de Brucella dans le rate de souris inocul\u00e9es par voie intrap\u00e9riton\u00e9ale","volume":"4","author":"Bosseray","year":"1976","journal-title":"J Biol Stand"},{"key":"10.1006\/biol.2000.0249_RF17","first-page":"257","article-title":"Theoretical, practical, and statistical basis for general control method of activity for anti- Brucella vaccines","volume":"56","author":"Bosseray","year":"1984","journal-title":"Dev Biol Stand"},{"key":"10.1006\/biol.2000.0249_RF18","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/0264-410X(90)90247-J","article-title":"B. suis S2, B. melitensis Rev 1 and B. abortus S19 living vaccines: residual virulence and immunity induced against three Brucella species challenge strains in mice","volume":"8","author":"Bosseray","year":"1990","journal-title":"Vaccine"},{"key":"10.1006\/biol.2000.0249_RF19","series-title":"Prevention of Brucellosis in Mediterranean Countries","first-page":"176","article-title":"Controle officiel du vaccin Rev 1 en France. Modalites\u2014Criteres\u2014Sanction","author":"Garin-Bastuji","year":"1992"},{"key":"10.1006\/biol.2000.0249_RF20","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0092-1157(77)80011-5","article-title":"Le controle des vaccins antibrucelliques par denombrement des Brucella dans la rate de souris, vaccinees ou non, inocul\u00e9es par voie intraperiton\u00e9ale","volume":"5","author":"Plommet","year":"1977","journal-title":"J Biol Stand"},{"key":"10.1006\/biol.2000.0249_RF21","first-page":"247","article-title":"Propositions pour un methode generale de controle d'activite des vaccins antibrucelliques","volume":"56","author":"Plommet","year":"1984","journal-title":"Dev Biol Stand"},{"key":"10.1006\/biol.2000.0249_RF22","first-page":"429","article-title":"Anti- Brucella cell-mediated immunity in mice vaccinated with cell-wall fraction","volume":"18","author":"Plommet","year":"1987","journal-title":"Ann Rech V\u00e9t"},{"key":"10.1006\/biol.2000.0249_RF23","unstructured":"O.I.E. Manual of Standards for Diagnostic tests and vaccines, 1996, Caprine and ovine brucellosis, 350, 362, Bovine brucellosis, 242, 255"},{"key":"10.1006\/biol.2000.0249_RF24","series-title":"Techniques for the brucellosis laboratories","author":"Alton","year":"1988"},{"key":"10.1006\/biol.2000.0249_RF25","first-page":"21","article-title":"La mesure statistique de la virulence et de I'immunit\u00e8. Application \u00e0 l'\u00e9tude de la virulence du bacille typhique et \u00e0 la mesure du pouvoir immunisant des vaccins antitypho\u0131\u0308diques","volume":"18","author":"Bonet-Maury","year":"1954","journal-title":"Rev Immunol"},{"key":"10.1006\/biol.2000.0249_RF26","series-title":"SAS\/STAT\u00aeUser's Guide, Version 6, Fourth Edition, Volume 2","year":"1989"},{"key":"10.1006\/biol.2000.0249_RF27","first-page":"759","article-title":"Characterization of Brucella abortus strain 19","volume":"33","author":"Brown","year":"1972","journal-title":"Am J Vet Res"},{"key":"10.1006\/biol.2000.0249_RF28","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/0264-410X(95)00214-L","article-title":"Improvement of the Brucella abortus B19 vaccine by its preparation in a glycerol based medium","volume":"14","author":"Sangari","year":"1996","journal-title":"Vaccine"},{"key":"10.1006\/biol.2000.0249_RF29","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/S0264-410X(98)00063-2","article-title":"The defect in the metabolism of erythritol of the Brucella abortus B19 vaccine strain is unrelated with its attenuated virulence in mice","volume":"16,17","author":"Sangari","year":"1998","journal-title":"Vaccine"},{"key":"10.1006\/biol.2000.0249_RF30","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1111\/j.1749-6632.1960.tb20106.x","article-title":"Biochemical properties of virulent and avirulent strains of Brucellae","volume":"88","author":"Wilson","year":"1960","journal-title":"Ann NY Acad Sci"},{"key":"10.1006\/biol.2000.0249_RF31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0769-2609(87)90086-X","article-title":"Brucellosis and immunity: humoral and cellular components in mice","volume":"183","author":"Plommet","year":"1987","journal-title":"Ann Inst. Pasteur, Microbiol"}],"container-title":["Biologicals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105600902495?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1045105600902495?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T03:36:42Z","timestamp":1576467402000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1045105600902495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["S1045105600902495"],"URL":"https:\/\/doi.org\/10.1006\/biol.2000.0249","relation":{},"ISSN":["1045-1056"],"issn-type":[{"value":"1045-1056","type":"print"}],"subject":[],"published":{"date-parts":[[2000,6]]}}}