{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T04:20:42Z","timestamp":1698380442969},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":6576,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The metabolism of [<jats:sup>3<\/jats:sup>H]labelled N\u2010Nitrosonornicotine a major constituent of the class of Tobacco Specific Nitrosamines was studied in adult and fetal human oesophageal cultures. The metabolites were separated by HPLC and were identified when compared to the standards as OH\u2010acid from 5\u2032hydroxylation, NNN\u20101\u2010N\u2010oxide formed via the pyridine N\u2010oxidation pathway and Keto acid from 2\u2032hydroxylation in both the adult and fetal cultures. These results indicate that hydroxylation which leads to electrophilic diazohyroxides is the major pathway of NNN metabolism in cultured human oesophagus. In the adult cultures levels of metabolites formed were 20.32 pmoles\/\u03bcg DNA of OH acid 11.08pmoles\/\u03bcg DNA of NNN\u20101\u2010N\u2010oxide and 7.67pmoles\/\u03bcg DNA of Keto acid. In the fetal cultures levels were 10.85pmoles\/\u03bcg DNA of OH acid, 9.40pmoles\/\u03bcg DNA of NNN\u20101\u2010N\u2010oxide and 7.91pmoles\/\u03bcg DNA of Keto acid. These results indicate that \u03b1\u2010hydroxylation is the key step in the metabolic activation of NNN in human oesophagus.<\/jats:p>","DOI":"10.1006\/cbir.1995.1007","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T22:25:04Z","timestamp":1033943104000},"page":"53-58","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Metabolism of N\u2032\u2010nitrosonornicotine by adult and fetal human oesophagal cultures"],"prefix":"10.1002","volume":"19","author":[{"given":"P. P.","family":"Chakradeo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Nair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. V.","family":"Bhide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1995.1007","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1995.1007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T20:49:28Z","timestamp":1698353368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1995.1007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1006\/cbir.1995.1007"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1995.1007","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}