{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:06:26Z","timestamp":1760886386344},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":5845,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>Propagation <jats:italic>in vitro<\/jats:italic> of rat tibial osteoblasts (ROB) is accompanied by increased expression of the early osteogenic marker alkaline phosphatase (AP) and maturation of the osteogenic phenotype. In order to establish the pattern of the integrin expressed in ROB during progression to the mature osteoblastic phenotype, we have used biosynthetic, immunoblotting and immuno\u2010histochemical assays. We immunoprecipitated from osteoblasts, expanded for 1.5\u2010 and 7.5\u2010doubling, \u03b15\u03b21, \u03b1v\u03b23, \u03b13\u03b21, \u03b16\u03b21 and \u03b11\u03b21 integrin heterodimers; furthermore \u03b25, \u03b12 and \u03b14 chains were detected by immunoblots and indirect immunofluorescence. \u03b1v, \u03b11, \u03b16 subunits in most cells, and \u03b23 and \u03b21 subunits in a minority, were found to be associated with adhesion plaques in osteoblasts of 1.5\u2010, 4.5\u2010 and 7.5\u2010doubling grown in the presence of FCS, while all other subunits stained diffusely all the cells. Adhesion to fibronectin (FN), laminin (LN), collagen type I (COL I) and III (COL III) by ROB at different doubling (1.5\u201311) was dependent on substratum concentration, and after 2.5h at 55nm 60% of the cells adhered to all substrata. Arg\u2010Gly\u2010Asp\u2010Ser (RGDS) containing peptides inhibited adhesion of cells differentially, according to substratum; no dependence on extent of progation <jats:italic>in vitro<\/jats:italic> was observed. In conclusion, ROB cultured <jats:italic>in vitro<\/jats:italic> for 1.5\u2010 to 11\u2010doubling had an unchanged pattern of expression of integrin subunits, heterodimer association and cellular distribution. Adhesion specificity and affinity were also unchanged. These results suggest that the phenotypic maturation, detected as an increase in AP expression, is not accompanied by major changes in the potential for cell\u2014matrix interactions, and does not correspond to changes in the type of integrin subunits expressed by osteoblasts.<\/jats:p>","DOI":"10.1006\/cbir.1996.0110","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T22:25:04Z","timestamp":1033943104000},"page":"7-16","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["OSTEOBLASTIC CELLS FROM RAT LONG BONE II: ADHESION TO SUBSTRATA AND INTEGRIN EXPRESSION IN PRIMARY AND PROPAGATED CULTURES"],"prefix":"10.1002","volume":"21","author":[{"given":"MIRCO","family":"CASTOLDI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIO","family":"PISTONE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CRISTINA","family":"CARUSO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALESSANDRA","family":"PUDDU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CRISTINA","family":"FILANTI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DANIELE","family":"PICCINI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CARLO","family":"TACCHETTI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PAOLA","family":"MANDUCA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1996.0110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1996.0110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T17:22:52Z","timestamp":1698427372000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1996.0110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1006\/cbir.1996.0110"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1996.0110","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}