{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T04:17:23Z","timestamp":1698466643513},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":5329,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p>To trace cell lineages and the origin and fate of cells in transplantation and embryo chimeras, a DNA\/DNA <jats:italic>in situ<\/jats:italic> hybridization cell labelling system was developed, based on a 50\u2010copy murine <jats:italic>c\u2010myc<\/jats:italic> transgene on mouse chromosome 8. Elevated levels of cMyc mRNA were found in Myc*tg50 (Myc<jats:sup>tg50<\/jats:sup>\/0 and Myc<jats:sup>tg50<\/jats:sup>\/Myc<jats:sup>tg50<\/jats:sup>) transgenic tissues, but adult transgenic NMRI mice were anatomically and histologically indistinguishable from control NMRI mice and did not develop tumours on a wild\u2010type or nude (<jats:italic>nu<\/jats:italic> \/ <jats:italic>nu<\/jats:italic>) background. The hybridization label detected transgenic nuclei with an efficiency of \u223c80%. In muscle grafts, this transgene label was successfully applied to trace donor cells in a labelled host and to study the invasion of a graft by host cells. When the cMyc hybridization was used in allophenic mice of the control|acNMRI\u2010Myc<jats:sup>tg50<\/jats:sup>\/? (<jats:italic>nu<\/jats:italic> \/+ or +\/+) type, an up to a three\u2010fold excess of MYC*tg50 positive over control nuclei was found in all organs examined (ventricle, skeletal muscle, liver, small intestine). This overgrowth of MYC*tg50 cells is probably due to transgene expression. Four out of seven (C57BL\/6\u00d7BALB\/c) or (C57BL\/6\u00d7NMRI)|acMYC*tg50 allophenic mice displayed anatomical abnormalities, e.g. an enlarged thymus and a tumour in the groin region. As these abnormalities were only observed in allophenic mice, they might be due to the imbalance of growth potential between MYC*tg50 transgenic and normal cells in the same individual.<\/jats:p>","DOI":"10.1006\/cbir.1998.0258","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T22:25:04Z","timestamp":1033943104000},"page":"401-411","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A MULTICOPY <i>c\u2010myc<\/i> TRANSGENE AS A NUCLEAR LABEL: OVERGROWTH OF Myc<sup>tg50<\/sup>CELLS IN ALLOPHENIC MICE"],"prefix":"10.1002","volume":"22","author":[{"given":"MARTIN","family":"AUGUSTIN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NORMAN","family":"KLOPP","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KAI","family":"EWALD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HARALD","family":"JOCKUSCH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1998.0258","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1998.0258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T22:47:09Z","timestamp":1698446829000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1998.0258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["10.1006\/cbir.1998.0258"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1998.0258","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}