{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:10:32Z","timestamp":1765545032891},"reference-count":0,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":5299,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:p>The effect of dexamethasone (DEX) on the expression of fibronectin (FN), pro\u03b1<jats:sub>1<\/jats:sub>(I) collagen (Col1), integrin \u03b1<jats:sub>2<\/jats:sub>, \u03b1<jats:sub>5<\/jats:sub>and \u03b2<jats:sub>1<\/jats:sub>subunits mRNAs, were studied by quantitative in situ hybridization (ISH) with radiolabelled probes in relationship with the organization of the extracellular matrix (ECM) of FN in human skin fibroblasts. In particular, two fibroblast strains were analysed, one derived from a control donor, typically organizing a rich ECM of FN, and the other from a patient affected by Ehlers\u2010Danlos syndrome (EDS), which did not assemble the FN\u2010ECM.<\/jats:p><jats:p>Treatment of both fibroblast strains with 10<jats:sup>\u22127<\/jats:sup><jats:sc>m<\/jats:sc>DEX slightly enhanced the level of FN mRNA (by about 1.5\u2010fold), did not influence the level of \u03b1<jats:sub>5<\/jats:sub>subunit mRNA and reduced Col1, \u03b1<jats:sub>2<\/jats:sub>and \u03b2<jats:sub>1<\/jats:sub>integrin subunits mRNAs by 2\u20133\u2010fold. These results show that, in these cells, DEX coordinately downregulates the expression of Col1 and its specific integrin \u03b1<jats:sub>2<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>. Moreover, DEX regulates in a different manner the \u03b1<jats:sub>5<\/jats:sub>and \u03b2<jats:sub>1<\/jats:sub>subunits forming the main FN receptor (FNR) in skin fibroblasts.<\/jats:p><jats:p>Immunofluorescence microscopy evidencing the FN\u2010ECM and integrins containing \u03b1<jats:sub>5<\/jats:sub>and \u03b2<jats:sub>1<\/jats:sub>subunits showed that in control cells DEX induced a slight enhancement of the FN\u2010ECM and of the \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>receptors patches. Therefore, in these cells the decrease of \u03b2<jats:sub>1<\/jats:sub>FN receptor subunit mRNA, as well as the decrease of Col1 and its receptor mRNAs, did not influence the FN\u2010ECM assembly. In EDS fibroblasts, DEX decreased the cytoplasmic accumulation of FN and induced the assembly of a rich FN\u2010ECM through the formation of large FNR integrin patches, codistributing with the FN\u2010ECM. We suggest that in EDS skin fibroblasts DEX corrects the defective FN\u2010ECM favouring the sorting and the organization of FN and its \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>integrin receptor.<\/jats:p>","DOI":"10.1006\/cbir.1998.0283","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T22:25:04Z","timestamp":1033943104000},"page":"499-508","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["EFFECT OF DEXAMETHASONE ON THE ASSEMBLY OF THE MATRIX OF FIBRONECTIN AND ON ITS RECEPTORS ORGANIZATION IN EHLERS\u2010DANLOS SYNDROME SKIN FIBROBLASTS"],"prefix":"10.1002","volume":"22","author":[{"given":"NICOLETTA","family":"ZOPPI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANNALISA","family":"GHINELLI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RITA","family":"GARDELLA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SERGIO","family":"BARLATI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARINA","family":"COLOMBI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1998.0283","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1998.0283","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T13:56:53Z","timestamp":1693403813000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1998.0283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7]]},"references-count":0,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[1998,7]]}},"alternative-id":["10.1006\/cbir.1998.0283"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1998.0283","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,7]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}