{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:04:57Z","timestamp":1790805897139,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":4750,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Replacement of L\u2010valine with D\u2010valine in a standard culture medium can selectively inhibit fibroblast proliferation. The aim of the present study was to investigate whether human myometrial cells cultured with D\u2010valine instead of L\u2010valine can survive and express their characteristics. Cultured cells (95\u201398%) maintain expression of the intermediate filament desmin, which is the specific marker for mature muscle cells. By transmission electron microscopy, the cells showed the general morphology of smooth muscle cells in culture. Oxytocin in serum\u2010free culture medium at 37\u00b0C (5min) caused a concentration\u2010dependent increase in cellular Na and total Ca, and a decrease in K content as determined by X\u2010ray microanalysis. The percentage of cells cultured with D\u2010valine responding to oxytocin stimulation was larger than that of cells cultured with L\u2010valine, suggesting less contamination of smooth muscle cells by fibroblasts in the presence of D\u2010valine. As shown by measurements with fura\u20102, D\u2010valine\u2010cultured cells retained the characteristic increase in intracellular free Ca<jats:sup>2+<\/jats:sup>ions after oxytocin stimulation.<\/jats:p>","DOI":"10.1006\/cbir.1999.0448","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T14:51:08Z","timestamp":1032187868000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["INHIBITION OF PROLIFERATION OF CONTAMINATING FIBROBLASTS BY D\u2010VALINE IN CULTURES OF SMOOTH MUSCLE CELLS FROM HUMAN MYOMETRIUM"],"prefix":"10.1002","volume":"24","author":[{"given":"Jarin","family":"Hongpaisan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1999.0448","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1999.0448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T07:07:24Z","timestamp":1698563244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1999.0448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1006\/cbir.1999.0448"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1999.0448","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}