{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:44:52Z","timestamp":1761216292066},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":4629,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ca<jats:sup>2+<\/jats:sup>mobilization induced by ATP, isoproterenol and the Ca<jats:sup>2+<\/jats:sup>\u2010ATPase inhibitor thapsigargin in the human submandibular duct cell line A253 was investigated using the Ca<jats:sup>2+<\/jats:sup>\u2010sensitive fluorescent indicator fura\u20102. ATP and isoproterenol increased cytosolic free Ca<jats:sup>2+<\/jats:sup>([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and subsequent exposure to thapsigargin after ATP or isoproterenol stimulation caused a further increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. However, ATP and isoproterenol were not able to elicit a further increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>after exposure of the cells to thapsigargin. Relatively few cells reacted to isoproterenol stimulation, but nearly all cells reacted to isoproterenol if ATP was added together with, or prior to isoproterenol stimulation. Moreover, the effect of ATP was potentiated by prior or simultaneous addition of isoproterenol. Furthermore, ATP decreased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in the presence of thapsigargin probably due to agonist\u2010induced export of intracellular calcium. The results may suggest the existence of three thapsigargin sensitive pools; one opened by ATP acting through P<jats:sub>2<\/jats:sub>\u2010purinergic receptors and IP<jats:sub>3<\/jats:sub>, one opened by isoproterenol acting through \u03b22\u2010adrenergic receptors, and a third pool not sensitive to ATP or isoproterenol.<\/jats:p>","DOI":"10.1006\/cbir.1999.0503","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T14:51:08Z","timestamp":1032187868000},"page":"273-277","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["CA<sup>2+<\/sup>MOBILIZATION IN THE HUMAN SUBMANDIBULAR DUCT CELL LINE A253"],"prefix":"10.1002","volume":"24","author":[{"given":"Charlotte","family":"Andersson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai\u2010Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Godfried M.","family":"Roomans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.1999.0503","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.1999.0503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T17:09:12Z","timestamp":1698599352000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.1999.0503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1006\/cbir.1999.0503"],"URL":"https:\/\/doi.org\/10.1006\/cbir.1999.0503","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}