{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:40:10Z","timestamp":1700419210977},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":4325,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p>The present study examined the involvement of protein kinase A (PKA), protein kinase G (PKG), protein kinase C (PKC), protein tyrosine kinase (PTK) and Ca<jats:sup>2+<\/jats:sup>\/calmodulin mediated pathways on the luminal uptake of L\u2010DOPA through the L\u2010type amino acid transporter in immortalized rat capillary cerebral endothelial (REB\u20104) cells. Non\u2010linear analysis of the saturation curve for L\u2010DOPA revealed a K<jats:sub>m<\/jats:sub>value (in \u03bcM) of 71\u00b19 and a V<jats:sub>max<\/jats:sub>value of 17\u00b11 (in nmol mg protein\/6min). L\u2010DOPA uptake at the luminal cell border was a sodium\u2010independent process and insensitive to N\u2010(methylamino)\u2010isobutyric acid (MeAIB, 1m <jats:sc>m<\/jats:sc>), but sensitive to 2\u2010aminobicyclo(2,2,1)\u2010heptane\u20102\u2010carboxylic acid (BHC, IC<jats:sub>50<\/jats:sub>=140\u03bc<jats:sc>m<\/jats:sc>). The Ca<jats:sup>2+<\/jats:sup>\/calmodulin inhibitors calmidazolium and trifluoperazine inhibited L\u2010DOPA (2.5\u03bc<jats:sc>m<\/jats:sc>) uptake with IC<jats:sub>50<\/jats:sub>s of 23 and 33\u03bc<jats:sc>m<\/jats:sc>, respectively. The inhibitory effect of BHC on the accumulation of L\u2010DOPA was of the competitive type, whereas that of calmidazolium and trifluoperazine was of the non\u2010competitive type. Modulators of PKA (cyclic AMP, forskolin, isobutylmethylxanthine and cholera toxin), PKG (cyclic GMP, zaprinast, LY 83583 and sodium nitroprusside), PKC (phorbol 12,13\u2010dibutyrate, staurosporine and chelerythrine) and PTK (genistein and tyrphostin 25) failed to affect the accumulation of a non\u2010saturating (2.5\u03bc<jats:sc>m<\/jats:sc>) concentration of L\u2010DOPA. It is concluded that L\u2010DOPA uptake in RBE\u20104 cells is promoted through the L\u2010type amino acid transporter and appears to be under the control of calmodulin mediated pathways.<\/jats:p>","DOI":"10.1006\/cbir.2000.0639","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T14:51:08Z","timestamp":1032187868000},"page":"245-252","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["INHIBITION OF CALCIUM\u2010INDEPENDENT LUMINAL UPTAKE OF L\u2010DOPA BY CALMODULIN ANTAGONISTS IN IMMORTALIZED RAT CAPILLARY CEREBRAL ENDOTHELIAL CELLS"],"prefix":"10.1002","volume":"25","author":[{"given":"B.","family":"Sampaio\u2010Maia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Soares\u2010Da\u2010Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.2000.0639","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.2000.0639","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:59:22Z","timestamp":1700416762000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.2000.0639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1006\/cbir.2000.0639"],"URL":"https:\/\/doi.org\/10.1006\/cbir.2000.0639","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}