{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T21:23:07Z","timestamp":1770499387909,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":4294,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:p>A view is emerging that human amylin (HA) kills pancreatic islet \u03b2\u2010cells by apoptosis. This study strengthens this view by documenting time\u2010dependent morphological and ultrastructural changes in 10\u03bcm HA\u2010treated cultured RINm5F islet \u03b2\u2010cells. Membrane blebbing and microvilli loss were the earliest detectable apoptosis\u2010related phenomena, already evident 1h after HA exposure. Following 6\u201312h of HA\u2010treatment, chromatin margination became evident, consistent with detecting DNA laddering about the same time. Nuclear shrinkage, nuclear membrane convolution and prominent cytoplasmic vacuolization were clearly recognized at 22h post\u2010treatment. Together, these cellular changes constitute a strong case for HA\u2010induced apoptosis, and further demonstrates that electron microscopy is a more sensitive tool for early apoptosis detection in cultured cells than classical biochemical assays like visualizing DNA laddering. The ultrastructural changes reported here contribute further evidence to be included in the ongoing dissection of molecular mechanisms underlying HA\u2010induced apoptosis, as may occur in type\u20102 diabetes mellitus.<\/jats:p>","DOI":"10.1006\/cbir.2000.0643","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T14:51:08Z","timestamp":1032187868000},"page":"339-350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["ULTRASTRUCTURAL EVIDENCE THAT APOPTOSIS IS THE MECHANISM BY WHICH HUMAN AMYLIN EVOKES DEATH IN RINm5F PANCREATIC ISLET \u03b2\u2010CELLS"],"prefix":"10.1002","volume":"25","author":[{"given":"Etuate L.","family":"Saafi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Konarkowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joerg","family":"Kistler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Garth J. S.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.2000.0643","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.2000.0643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T09:27:17Z","timestamp":1700299637000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.2000.0643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,4]]}},"alternative-id":["10.1006\/cbir.2000.0643"],"URL":"https:\/\/doi.org\/10.1006\/cbir.2000.0643","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}