{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T14:40:03Z","timestamp":1700232003258},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T00:00:00Z","timestamp":1357084800000},"content-version":"vor","delay-in-days":3685,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study examined the effect of Bak Foong Pills (BFP), an over\u2010the\u2010counter traditional Chinese medicine (China registration no. Z980035), on anion secretion and the underlying signaling pathways in normal and cystic fibrosis pancreatic duct cell lines, CAPAN\u20101 and CFPAC\u20101, respectively, using the short\u2010circuit current technique. Apical addition of BFP ethanol extract (600\u03bcg\/ml) induced a fast transient I<jats:sub>SC<\/jats:sub> peak that was followed by a slower but more sustained increase in I<jats:sub>SC<\/jats:sub> in CAPAN\u20101 cells. However, the response to BFP in CFPAC\u20101 was predominantly the first transient peak. Apical addition of DIDS (200\u03bcM) inhibited the first peak by more than 60% in both cell lines without significantly affecting the second I<jats:sub>SC<\/jats:sub> rise. More than 85% of the BFP\u2010induced first transient in both cell lines was inhibited when extra and intracellular Ca<jats:sup>2+<\/jats:sup> was chelated or emptied by pre\u2010treatment with BAPTA (100\u03bcM) and thapsigargin (10\u03bcM), respectively. Acute addition of PMA (1\u03bcM), a PKC activator, blocked more than 95% of the BFP\u2010induced first peak in both cell lines, consistent with previously reported PKC modulation of Ca<jats:sup>2+<\/jats:sup>\u2010dependent pancreatic anion secretion. The BFP\u2010induced second I<jats:sub>SC<\/jats:sub> rise in CAPAN\u20101 could be inhibited by 73.6% and 71.13% by pretreatment of the cells with MDL\u201012330A (20\u03bcM), an adenylate cyclase inhibitor and Rp\u2010cAMP (200\u03bcM), a cyclic AMP antagonist, respectively. However, less than 25% of the I<jats:sub>SC<\/jats:sub> was inhibited by combined treatment with BAPTA and thapsigargin. The second rise was also completely blocked by DPC (2mM) or Glibenclamide (1mM). The results indicate that BFP ethanol extract stimulates pancreatic duct anion secretion in normal and CF cells via different signaling pathways involving both Ca<jats:sup>2+<\/jats:sup> and cAMP.<\/jats:p>","DOI":"10.1006\/cbir.2002.0960","type":"journal-article","created":{"date-parts":[[2002,12,10]],"date-time":"2002-12-10T20:43:20Z","timestamp":1039553000000},"page":"1011-1018","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["BAK FOONG PILLS STIMULATE ANION SECRETION ACROSS NORMAL AND CYSTIC FIBROSIS PANCREATIC DUCT EPITHELIA"],"prefix":"10.1002","volume":"26","author":[{"given":"Jin Xia","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pui Shan","family":"Lo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen Chao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning Chao","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dewi K.","family":"Rowlands","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu Lin","family":"Gou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiu Wa","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiao Chang","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,2]]},"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1006%2Fcbir.2002.0960","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1006\/cbir.2002.0960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T14:19:55Z","timestamp":1700230795000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1006\/cbir.2002.0960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1006\/cbir.2002.0960"],"URL":"https:\/\/doi.org\/10.1006\/cbir.2002.0960","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12]]},"assertion":[{"value":"2013-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}