{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:20:38Z","timestamp":1791217238454,"version":"4.1.0"},"reference-count":63,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":4672,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Biology"],"published-print":{"date-parts":[[2000,10]]},"DOI":"10.1006\/dbio.2000.9847","type":"journal-article","created":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T15:45:48Z","timestamp":1032191148000},"page":"152-165","source":"Crossref","is-referenced-by-count":49,"title":["The NED-8 Conjugating System in Caenorhabditis elegans Is Required for Embryogenesis and Terminal Differentiation of the Hypodermis"],"prefix":"10.1016","volume":"226","author":[{"given":"Donald","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.Peter M","family":"Candido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1006\/dbio.2000.9847_RF1","series-title":"Current Protocols in Molecular Biology","author":"Ausubel","year":"1987"},{"key":"10.1006\/dbio.2000.9847_RF2","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1242\/dev.124.21.4333","article-title":"The terminal differentiation factor LIN-29 is required for proper vulval morphogenesis and egg laying in Caenorhabditis elegans","volume":"124","author":"Bettinger","year":"1997","journal-title":"Development"},{"key":"10.1006\/dbio.2000.9847_RF3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0092-8674(94)90396-4","article-title":"The ubiquitin\u2013proteasome proteolytic pathway","volume":"79","author":"Ciechanover","year":"1994","journal-title":"Cell"},{"key":"10.1006\/dbio.2000.9847_RF4","doi-asserted-by":"crossref","first-page":"15342","DOI":"10.1073\/pnas.96.26.15342","article-title":"The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1","volume":"96","author":"del Pozo","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF5","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1126\/science.280.5370.1760","article-title":"The ubiquitin-related protein RUB1 and auxin response in Arabidopsis","volume":"280","author":"del Pozo","year":"1998","journal-title":"Science"},{"key":"10.1006\/dbio.2000.9847_RF6","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1006\/dbio.1998.9063","article-title":"The LIN-29 transcription factor is required for proper morphogenesis of the Caenorhabditis elegans male tail","volume":"206","author":"Euling","year":"1999","journal-title":"Dev. Biol."},{"key":"10.1006\/dbio.2000.9847_RF7","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0092-8674(00)80404-3","article-title":"A complex of Cdc4p, Skp1p, and Cdc53p\/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p","volume":"91","author":"Feldman","year":"1997","journal-title":"Cell"},{"key":"10.1006\/dbio.2000.9847_RF8","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/70272","article-title":"CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans","volume":"1","author":"Feng","year":"1999","journal-title":"Nat. Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF9","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","article-title":"Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans","volume":"391","author":"Fire","year":"1998","journal-title":"Nature"},{"key":"10.1006\/dbio.2000.9847_RF10","first-page":"387","article-title":"Degradation of an ubiquitin-conjugated protein is associated with myoblast differentiation in primary cell culture","volume":"47","author":"Gardrat","year":"1999","journal-title":"Biochem. Mol. Biol. Int."},{"key":"10.1006\/dbio.2000.9847_RF11","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1126\/science.2842867","article-title":"The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme","volume":"241","author":"Goebl","year":"1988","journal-title":"Science"},{"key":"10.1006\/dbio.2000.9847_RF12","doi-asserted-by":"crossref","first-page":"12036","DOI":"10.1074\/jbc.274.17.12036","article-title":"Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway","volume":"274","author":"Gong","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF13","doi-asserted-by":"crossref","first-page":"11315","DOI":"10.1016\/S0021-9258(18)60961-5","article-title":"Interferon induces a 15-kilodalton protein exhibiting marked homology to ubiquitin","volume":"262","author":"Haas","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF14","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1096\/fasebj.11.14.9409544","article-title":"Pathways of ubiquitin conjugation","volume":"11","author":"Haas","year":"1997","journal-title":"FASEB J."},{"key":"10.1006\/dbio.2000.9847_RF15","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1146\/annurev.bi.61.070192.003553","article-title":"The ubiquitin system for protein degradation","volume":"61","author":"Hershko","year":"1992","journal-title":"Annu. Rev. Biochem."},{"key":"10.1006\/dbio.2000.9847_RF16","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1046\/j.1365-313X.1995.7020211.x","article-title":"The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism and lateral root initiation","volume":"7","author":"Hobbie","year":"1995","journal-title":"Plant J."},{"key":"10.1006\/dbio.2000.9847_RF17","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev.genet.30.1.405","article-title":"Ubiquitin-dependent protein degradation","volume":"30","author":"Hochstrasser","year":"1996","journal-title":"Annu. Rev. Genet."},{"key":"10.1006\/dbio.2000.9847_RF18","doi-asserted-by":"crossref","first-page":"28766","DOI":"10.1074\/jbc.271.46.28766","article-title":"The tail of a ubiquitin-conjugating enzyme redirects multi-ubiquitin chain synthesis from the lysine 48-linked configuration to a novel nonlysine-linked form","volume":"271","author":"Hodgins","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF19","doi-asserted-by":"crossref","first-page":"6829","DOI":"10.1038\/sj.onc.1203093","article-title":"Covalent modification of all members of human cullin family proteins by NEDD8","volume":"18","author":"Hori","year":"1999","journal-title":"Oncogene"},{"key":"10.1006\/dbio.2000.9847_RF20","doi-asserted-by":"crossref","first-page":"12436","DOI":"10.1073\/pnas.96.22.12436","article-title":"Identification of the von Hippel\u2013Lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex","volume":"96","author":"Iwai","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF21","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/S0960-9822(99)80333-9","article-title":"Depletion of syntaxins in the early Caenorhabditis elegans embryo reveals a role for membrane fusion events in cytokinesis","volume":"9","author":"Jantsch-Plunger","year":"1999","journal-title":"Curr. Biol."},{"key":"10.1006\/dbio.2000.9847_RF22","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/S0962-8924(97)01132-X","article-title":"SUMO-1: Ubiquitin gains weight","volume":"7","author":"Johnson","year":"1997","journal-title":"Trends Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF23","doi-asserted-by":"crossref","first-page":"28557","DOI":"10.1074\/jbc.272.45.28557","article-title":"Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein","volume":"272","author":"Kamitani","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF24","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1126\/science.284.5414.657","article-title":"Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase","volume":"284","author":"Kamura","year":"1999","journal-title":"Science"},{"key":"10.1006\/dbio.2000.9847_RF25","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0092-8674(00)81267-2","article-title":"cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family","volume":"85","author":"Kipreos","year":"1996","journal-title":"Cell"},{"key":"10.1006\/dbio.2000.9847_RF26","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1128\/MCB.10.5.2081","article-title":"The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen","volume":"10","author":"Kramer","year":"1990","journal-title":"Mol. Cell. Biol."},{"key":"10.1006\/dbio.2000.9847_RF27","series-title":"Cell and Molecular Biology of the Cytoskeleton","first-page":"151","article-title":"The actin genes in Caenorhabditis elegans","author":"Krause","year":"1986"},{"key":"10.1006\/dbio.2000.9847_RF28","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/0092-8674(87)90613-1","article-title":"A trans-spliced leader sequence on actin mRNA in C. elegans","volume":"49","author":"Krause","year":"1987","journal-title":"Cell"},{"key":"10.1006\/dbio.2000.9847_RF29","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1006\/bbrc.1993.2056","article-title":"Cloning of a cDNA which encodes a novel ubiquitin-like protein","volume":"195","author":"Kumar","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1006\/dbio.2000.9847_RF30","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1101\/gad.12.7.914","article-title":"Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCFCdc4 complex","volume":"12","author":"Lammer","year":"1998","journal-title":"Genes Dev."},{"key":"10.1006\/dbio.2000.9847_RF31","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1042\/bj3270357","article-title":"Biochemical characterization of Caenorhabditis elegans UBC-1: Self-association and auto-ubiquitination of a RAD6-like ubiquitin-conjugating enzyme in vitro","volume":"327","author":"Leggett","year":"1997","journal-title":"Biochem. J."},{"key":"10.1006\/dbio.2000.9847_RF32","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1089\/dna.1995.14.883","article-title":"Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6\/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes","volume":"14","author":"Leggett","year":"1995","journal-title":"DNA Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF33","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1006\/dbio.1999.9540","article-title":"Double-stranded RNA injection produces null phenotypes in zebrafish","volume":"217","author":"Li","year":"2000","journal-title":"Dev. Biol."},{"key":"10.1006\/dbio.2000.9847_RF34","doi-asserted-by":"crossref","first-page":"5510","DOI":"10.1073\/pnas.96.10.5510","article-title":"Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel\u2013Lindau tumor suppressor function","volume":"96","author":"Liakopoulos","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF35","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1093\/emboj\/17.8.2208","article-title":"A novel protein modification pathway related to the ubiquitin system","volume":"17","author":"Liakopoulos","year":"1998","journal-title":"EMBO J."},{"key":"10.1006\/dbio.2000.9847_RF36","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1101\/gad.13.14.1822","article-title":"The von Hippel\u2013Lindau tumor suppressor protein is a component of an E3 ubiquitin-protein ligase activity","volume":"13","author":"Lisztwan","year":"1999","journal-title":"Genes Dev."},{"key":"10.1006\/dbio.2000.9847_RF37","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1093\/emboj\/17.2.368","article-title":"Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): Evidence for evolutionary conservation in the subunit composition of the CDC34\u2013SCF pathway","volume":"17","author":"Lisztwan","year":"1998","journal-title":"EMBO J."},{"key":"10.1006\/dbio.2000.9847_RF38","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1242\/dev.121.8.2471","article-title":"The Caenorhabditis elegans heterochronic gene pathway controls stage-specific transcription of collagen genes","volume":"121","author":"Liu","year":"1995","journal-title":"Development"},{"key":"10.1006\/dbio.2000.9847_RF39","doi-asserted-by":"crossref","first-page":"8408","DOI":"10.1128\/MCB.14.12.8408","article-title":"Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern","volume":"14","author":"Loeb","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"10.1006\/dbio.2000.9847_RF40","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0092-8674(00)81862-0","article-title":"A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2","volume":"88","author":"Mahajan","year":"1997","journal-title":"Cell"},{"key":"10.1006\/dbio.2000.9847_RF41","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1083\/jcb.135.6.1457","article-title":"A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex","volume":"135","author":"Matunis","year":"1996","journal-title":"J. Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF42","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1083\/jcb.140.3.499","article-title":"SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex","volume":"140","author":"Matunis","year":"1998","journal-title":"J. Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF43","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/ng0592-124","article-title":"Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues","volume":"1","author":"McCombie","year":"1992","journal-title":"Nat. Genet."},{"key":"10.1006\/dbio.2000.9847_RF44","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1083\/jcb.147.3.471","article-title":"HCP-1, a protein involved in chromosome segregation, is localized to the centromere of mitotic chromosomes in Caenorhabditis elegans","volume":"147","author":"Moore","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1006\/dbio.2000.9847_RF45","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1074\/jbc.271.1.324","article-title":"Conjugation of the 15-kDa interferon-induced ubiquitin homolog is distinct from that of ubiquitin","volume":"271","author":"Narasimhan","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF46","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.1242\/dev.122.11.3617","article-title":"Morphogenesis of the C. elegans hermaphrodite uterus","volume":"122","author":"Newman","year":"1996","journal-title":"Development"},{"key":"10.1006\/dbio.2000.9847_RF47","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/312663a0","article-title":"The yeast ubiquitin gene: Head-to-tail repeats encoding a polyubiquitin precursor protein","volume":"312","author":"Ozkaynak","year":"1984","journal-title":"Nature"},{"key":"10.1006\/dbio.2000.9847_RF48","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1101\/gad.12.5.692","article-title":"Cdc53 is a scaffold protein for multiple Cdc34\/Skp1\/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast","volume":"12","author":"Patton","year":"1998","journal-title":"Genes Dev."},{"key":"10.1006\/dbio.2000.9847_RF49","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/prot.340190108","article-title":"Combining evolutionary information and neural networks to predict protein secondary structure","volume":"19","author":"Rost","year":"1994","journal-title":"Proteins"},{"key":"10.1006\/dbio.2000.9847_RF50","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1242\/dev.121.8.2491","article-title":"The heterochronic gene lin-29 encodes a zinc finger protein that controls a terminal differentiation event in Caenorhabditis elegans","volume":"121","author":"Rougvie","year":"1995","journal-title":"Development"},{"key":"10.1006\/dbio.2000.9847_RF51","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1006\/dbio.2000.9847_RF52","doi-asserted-by":"crossref","first-page":"12148","DOI":"10.1074\/jbc.273.20.12148","article-title":"Characterization of human hect domain family members and their interaction with UbcH5 and UbcH7","volume":"273","author":"Schwarz","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF53","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1242\/dev.126.4.691","article-title":"Formation of the vulva in Caenorhabditis elegans: A paradigm for organogenesis","volume":"126","author":"Sharma-Kishore","year":"1999","journal-title":"Development"},{"key":"10.1006\/dbio.2000.9847_RF54","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1093\/nar\/25.6.1162","article-title":"Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance","volume":"25","author":"Shayeghi","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1006\/dbio.2000.9847_RF55","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/S0960-9822(98)70465-8","article-title":"The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos","volume":"8","author":"Skop","year":"1998","journal-title":"Curr. Biol."},{"key":"10.1006\/dbio.2000.9847_RF56","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1093\/nar\/26.1.320","article-title":"Pfam: Multiple sequence alignments and HMM-profiles of protein domains","volume":"26","author":"Sonnhammer","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1006\/dbio.2000.9847_RF57","doi-asserted-by":"crossref","first-page":"3191","DOI":"10.1101\/gad.13.24.3191","article-title":"Targeted mRNA degradation by double-stranded RNA in vitro","volume":"13","author":"Tuschl","year":"1999","journal-title":"Genes Dev."},{"key":"10.1006\/dbio.2000.9847_RF58","doi-asserted-by":"crossref","first-page":"12230","DOI":"10.1073\/pnas.96.22.12230","article-title":"VHL: A very hip ligase","volume":"96","author":"Tyers","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF59","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.1073\/pnas.97.9.4499","article-title":"Caenorhabditis elegans embryonic axial patterning requires two recently discovered posterior-group Hox genes","volume":"97","author":"Van Auken","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF60","doi-asserted-by":"crossref","first-page":"36025","DOI":"10.1074\/jbc.274.50.36025","article-title":"The von Hippel\u2013Lindau tumor suppressor gene product promotes, but is not essential for, NEDD8 conjugation to cullin-2","volume":"274","author":"Wada","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2000.9847_RF61","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1006\/bbrc.1999.0339","article-title":"Identification of NEDD8-conjugation site in human cullin-2","volume":"257","author":"Wada","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1006\/dbio.2000.9847_RF62","doi-asserted-by":"crossref","first-page":"10836","DOI":"10.1073\/pnas.92.24.10836","article-title":"The genome of Caenorhabditis elegans","volume":"92","author":"Waterston","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2000.9847_RF63","series-title":"The Nematode Caenorhabditis elegans","first-page":"81","article-title":"The anatomy","author":"White","year":"1988"}],"container-title":["Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160600998476?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160600998476?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T02:13:44Z","timestamp":1620008024000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012160600998476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["S0012160600998476"],"URL":"https:\/\/doi.org\/10.1006\/dbio.2000.9847","relation":{},"ISSN":["0012-1606"],"issn-type":[{"value":"0012-1606","type":"print"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}