{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T01:59:44Z","timestamp":1778551184673,"version":"3.51.4"},"reference-count":43,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":4368,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Biology"],"published-print":{"date-parts":[[2001,8]]},"DOI":"10.1006\/dbio.2001.0314","type":"journal-article","created":{"date-parts":[[2003,4,24]],"date-time":"2003-04-24T17:03:32Z","timestamp":1051203812000},"page":"210-219","source":"Crossref","is-referenced-by-count":33,"title":["Calmodulin Antagonists Inhibit T-Type Ca2+ Currents in Mouse Spermatogenic Cells and the Zona Pellucida-Induced Sperm Acrosome Reaction"],"prefix":"10.1016","volume":"236","author":[{"given":"Ignacio","family":"L\u00f3pez-Gonz\u00e1lez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose L.","family":"De La Vega-Beltr\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia M.","family":"Santi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey M.","family":"Florman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Felix","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Darszon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/dbio.2001.0314_RF1","doi-asserted-by":"crossref","first-page":"13004","DOI":"10.1073\/pnas.93.23.13004","article-title":"Activation of mouse sperm T-type Ca2+ channels by adhesion to the egg zona pellucida","volume":"93","author":"Arnoult","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2001.0314_RF2","doi-asserted-by":"crossref","first-page":"6757","DOI":"10.1073\/pnas.96.12.6757","article-title":"Control of the low voltage-activated calcium channel of mouse sperm by egg ZP3 and by membrane hyperpolarization during capacitation","volume":"96","author":"Arnoult","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2001.0314_RF3","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1093\/emboj\/16.7.1593","article-title":"Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation","volume":"16","author":"Arnoult","year":"1997","journal-title":"EMBO J."},{"key":"10.1006\/dbio.2001.0314_RF4","first-page":"1104","article-title":"Pharmacological properties of the T-type Ca2+ current of mouse spermatogenic cells","volume":"53","author":"Arnoult","year":"1998","journal-title":"Mol. Pharmacol."},{"key":"10.1006\/dbio.2001.0314_RF5","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1083\/jcb.134.3.637","article-title":"ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization","volume":"134","author":"Arnoult","year":"1996","journal-title":"J. Cell. Biol."},{"key":"10.1006\/dbio.2001.0314_RF6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1017\/S0967199499000441","article-title":"Calmodulin, gametes and fertilisation","volume":"7","author":"Courtot","year":"1999","journal-title":"Zygote"},{"key":"10.1006\/dbio.2001.0314_RF7","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1095\/biolreprod41.4.635","article-title":"Methods for evaluating the acrosomal status of mammalian sperm","volume":"41","author":"Cross","year":"1989","journal-title":"Biol. Reprod."},{"key":"10.1006\/dbio.2001.0314_RF8","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1152\/physrev.1999.79.2.481","article-title":"Ion channels in sperm physiology","volume":"79","author":"Darszon","year":"1999","journal-title":"Physiol. Rev."},{"key":"10.1006\/dbio.2001.0314_RF9","doi-asserted-by":"crossref","first-page":"5718","DOI":"10.1073\/pnas.85.15.5718","article-title":"Paramecium calcium channels are blocked by a family of calmodulin antagonists","volume":"85","author":"Ehrlich","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2001.0314_RF10","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0014-5793(98)00305-6","article-title":"Mouse sperm patch-clamp recordings reveal single Cl\u2212 channels sensitive to niflumic acid, a blocker of the sperm acrosome reaction","volume":"426","author":"Espinosa","year":"1998","journal-title":"FEBS Lett."},{"key":"10.1006\/dbio.2001.0314_RF11","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0304-4157(00)00018-6","article-title":"Dynamics of the mammalian sperm plasma membrane in the process of fertilization","volume":"1469","author":"Flesch","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/dbio.2001.0314_RF12","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0005-2736(89)90206-X","article-title":"Egg jelly triggers a calcium influx which inactivates and is inhibited by calmodulin antagonists in the sea urchin sperm","volume":"980","author":"Guerrero","year":"1989","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/dbio.2001.0314_RF13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1113\/jphysiol.1984.sp015457","article-title":"Calcium and potassium currents in spermatogenic cells dissociated from rat seminiferous tubules","volume":"356","author":"Hagiwara","year":"1984","journal-title":"J. Physiol."},{"key":"10.1006\/dbio.2001.0314_RF14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF00656997","article-title":"Improved patch-clamp techniques for high-resolution current recording from cells and cell free membrane patches","volume":"391","author":"Hamill","year":"1981","journal-title":"Pfluegers Arch."},{"key":"10.1006\/dbio.2001.0314_RF15","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/0040-8166(94)90036-1","article-title":"Calmodulin binding proteins in the membrane vesicles released during the acrosome reaction and in the perinuclear material in isolated acrosome reacted sperm heads","volume":"26","author":"Hern\u00e1ndez","year":"1994","journal-title":"Tissue Cell"},{"key":"10.1006\/dbio.2001.0314_RF16","series-title":"Ionic Channels of Excitable Membranes","author":"Hille","year":"1992"},{"key":"10.1006\/dbio.2001.0314_RF17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/BFb0033648","article-title":"Voltage-dependent calcium channels: From structure to function","volume":"139","author":"Hofmann","year":"1999","journal-title":"Rev. Physiol. Biochem. Pharmacol."},{"key":"10.1006\/dbio.2001.0314_RF18","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF01868532","article-title":"Anticalmodulin drugs block the sodium gating current of squid giant axons","volume":"120","author":"Ichikawa","year":"1991","journal-title":"J. Membr. Biol."},{"key":"10.1006\/dbio.2001.0314_RF19","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.1073\/pnas.77.5.2772","article-title":"Calmodulin localization in mammalian spermatozoa","volume":"77","author":"Jones","year":"1980","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2001.0314_RF20","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1002\/ar.1092300407","article-title":"Localization of calmodulin in perinuclear structures of spermatids and spermatozoa: A comparison of six mammalian species","volume":"230","author":"Kann","year":"1991","journal-title":"Anat. Rec."},{"key":"10.1006\/dbio.2001.0314_RF21","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1476-5381.1994.tb14760.x","article-title":"Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists","volume":"111","author":"Kleene","year":"1994","journal-title":"Br. J. Pharmacol."},{"key":"10.1006\/dbio.2001.0314_RF22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s002329900179","article-title":"The actions of calmodulin antagonists W-7 and TFP and of calcium on the gating kinetics of the calcium-activated large conductance potassium channel of the chara protoplasmic drop: A substate-sensitive analysis","volume":"155","author":"Laver","year":"1997","journal-title":"J. Membr. Biol."},{"key":"10.1006\/dbio.2001.0314_RF23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/20194","article-title":"Ca2+\/calmodulin binds to and modulates P\/Q-type calcium channels","volume":"399","author":"Lee","year":"1999","journal-title":"Nature"},{"key":"10.1006\/dbio.2001.0314_RF24","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1095\/biolreprod33.1.235","volume":"33","author":"Lee","year":"1985","journal-title":"Biol. Reprod."},{"key":"10.1006\/dbio.2001.0314_RF25","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/0014-5793(96)00515-7","article-title":"T-type Ca2+ channels and \u03b11E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction","volume":"388","author":"Li\u00e9vano","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1006\/dbio.2001.0314_RF26","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1021\/bi00455a012","article-title":"Trifluoperazine binding to porcine brain calmodulin and skeletal muscle troponin C","volume":"29","author":"Massom","year":"1990","journal-title":"Biochemistry"},{"key":"10.1006\/dbio.2001.0314_RF27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.1982.62.1.1","article-title":"Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells","volume":"62","author":"Means","year":"1982","journal-title":"Physiol. Rev."},{"key":"10.1006\/dbio.2001.0314_RF28","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/S0896-6273(00)80709-6","article-title":"Calmodulin is the Ca2+ sensor for Ca2+-dependent inactivation of L-type calcium channels","volume":"22","author":"Peterson","year":"1999","journal-title":"Neuron"},{"key":"10.1006\/dbio.2001.0314_RF29","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1073\/pnas.96.5.2435","article-title":"Ca2+-induced inhibition of the cardiac Ca2+ channel depends on calmodulin","volume":"96","author":"Qin","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/dbio.2001.0314_RF30","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1096\/fasebj.11.5.9141499","article-title":"Sequence motifs for calmodulin recognition","volume":"11","author":"Rhoads","year":"1997","journal-title":"FASEB J."},{"key":"10.1006\/dbio.2001.0314_RF31","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1002\/(SICI)1098-2795(199912)54:4<418::AID-MRD13>3.0.CO;2-I","article-title":"Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca2+ complex in mouse sperm for use in estimating intracellular Ca2+ concentrations","volume":"54","author":"Rockwell","year":"1999","journal-title":"Mol. Reprod. Dev."},{"key":"10.1006\/dbio.2001.0314_RF32","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/jez.1402260318","article-title":"Inhibition of the acrosome reaction of sea urchin spermatozoa by a calmodulin antagonist, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7)","volume":"226","author":"Sano","year":"1983","journal-title":"J. Exp. Zool."},{"key":"10.1006\/dbio.2001.0314_RF33","doi-asserted-by":"crossref","first-page":"C1583","DOI":"10.1152\/ajpcell.1996.271.5.C1583","article-title":"A dihydropyridine-sensitive T-type Ca2+ current is the main Ca2+ current carrier in mouse primary spermatocytes","volume":"271","author":"Santi","year":"1996","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1006\/dbio.2001.0314_RF34","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1042\/bj3130661","article-title":"Calmidazolium leads to an increase in the cytosolic Ca2+ concentration in Dictyostelium discoideum by induction of Ca2+ release from intracellular stores and influx of extracellular Ca2+","volume":"313","author":"Schlatterer","year":"1996","journal-title":"Biochem. J."},{"key":"10.1006\/dbio.2001.0314_RF35","doi-asserted-by":"crossref","first-page":"5516","DOI":"10.1210\/endo.140.12.7180","article-title":"Ca2+\/calmodulin inhibition and phospholipase C-linked Ca2+ signaling in clonal beta-cells","volume":"140","author":"Schofl","year":"1999","journal-title":"Endocrinology"},{"key":"10.1006\/dbio.2001.0314_RF36","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1085\/jgp.114.2.185","article-title":"State-dependent inactivation of the \u03b11G T-type calcium channel","volume":"114","author":"Serrano","year":"1999","journal-title":"J. Gen. Physiol."},{"key":"10.1006\/dbio.2001.0314_RF37","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1095\/biolreprod62.5.1231","article-title":"Evidence for the involvement of calmodulin in mouse sperm capacitation","volume":"62","author":"Si","year":"2000","journal-title":"Biol. Reprod."},{"key":"10.1006\/dbio.2001.0314_RF38","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/S0968-0004(99)01383-3","article-title":"The Ca-calmodulin-dependent protein kinase cascade","volume":"24","author":"Soderling","year":"1999","journal-title":"Trends Biochem. Sci."},{"key":"10.1006\/dbio.2001.0314_RF39","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1074\/jbc.274.5.3235","article-title":"Cholesterol efflux-mediated signal transduction in mammalian sperm. \u03b2-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation","volume":"274","author":"Visconti","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1006\/dbio.2001.0314_RF40","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0092-8674(00)80558-9","article-title":"Mammalian fertilization: Molecular aspects of gamete adhesion, exocytosis, and fusion","volume":"96","author":"Wassarman","year":"1999","journal-title":"Cell"},{"key":"10.1006\/dbio.2001.0314_RF41","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1038\/368859a0","article-title":"Cloning and functional expression of a cyclic-nucleotide-gated channel from mammalian sperm","volume":"368","author":"Weyand","year":"1994","journal-title":"Nature"},{"key":"10.1006\/dbio.2001.0314_RF42","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/20200","article-title":"Calmodulin supports both inactivation and facilitation of L-type calcium channels","volume":"399","author":"Z\u00fchlke","year":"1999","journal-title":"Nature"},{"key":"10.1006\/dbio.2001.0314_RF43","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.1073\/pnas.95.6.3287","article-title":"Ca2+-sensitive inactivation of L-type Ca2+ channels depends on multiple cytoplasmic amino acid sequences of the \u03b11C subunit","volume":"95","author":"Z\u00fchlke","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160601903148?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0012160601903148?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T18:19:48Z","timestamp":1576520388000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0012160601903148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["S0012160601903148"],"URL":"https:\/\/doi.org\/10.1006\/dbio.2001.0314","relation":{},"ISSN":["0012-1606"],"issn-type":[{"value":"0012-1606","type":"print"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}